Naslov
Effects of heavy metal pollution on pigmented macrophages in kidney of Vardar chub (Squalius vardarensis Karaman)

Autori
Jordanova, Maja ; Rebok, Katerina ; Dragun, Zrinka ; Ramani, Sheriban ; Ivanova, Lozenka ; Kostov, Vasil ; Valić, Damir ; Krasnići, Nesrete ; Filipović Marijić, Vlatka ; Kapetanović, Damir

Izvornik
Microscopy research and technique (1059-910X) 80 (2017), 8; 930-935

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
heavy metals ; macrophage ; pigments ; river pollution ; trunk kidney ; Vardar chub

Sažetak
Freshwater pollution with heavy metals may influence the immune system of fish, leading to impairment of their health or even to increase of their mortality. The fish kidney is one of the first fish organs to be affected by water contamination. Amounts of kidney macrophages (MACs), which are involved in fish immune response, as well as the qualitative and quantitative changes in the pigmented MACs in fish kidney, are used as biomarkers of pollution. Therefore, in this study we have evaluated relative and total volumes of trunk kidney pigmented MACs, and analyzed the pigments accumulated within them, in Vardar chub (Squalius vardarensis Karaman) sampled from two mining impacted rivers, Kriva and Zletovska, highly contaminated with heavy metals, and from one reference river, Bregalnica, in spring and autumn of 2012. We have observed that main pigments found in kidney MACs of Vardar chub were melanin and lipofuscin/ceroid, as well as that relative volumes of MACs ranged from 0.56-1.68%. Moreover, the results showed that relative volumes of pigmented MACs were higher in metal contaminated rivers, especially in autumn season in the Zletovska River, concurrently with extremely high metal exposure. In addition, condition factors and kidney somatic indices were found significantly lower in the Zletovska River in both seasons, autumn and spring, possibly also as a consequence of high water pollution. Our data confirm that increase in relative volumes of pigmented MACs may serve as warning sign of potential heavy metal pollution in aquatic environment.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA