Naslov
Immunogenicity of synthetic peptides of measles virus origin and influence of peptidoglycan monomer as adjuvant

Autori
Mateljak, Sanja ; Halassy Špoljar, Beata ; Dojnović, Biserka ; Bouche, F. Fabienne ; Mutz, M. Mike ; Muller, P. Claude ; Tomašić, Jelka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2001 Annual Meeting of the Croatian Immunological Society : Programme, Abstracts / - Zagreb : Hrvatsko imunološko društvo, 2001, 10-10

Skup
2001 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Zagreb, Hrvatska, 07.12.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Sažetak
Synthetic peptides comprising critical epitopes from the measles virus (MV) have a great potential as vaccines1. Peptidoglycan monomer (PGM) is a disaccharide pentapeptide originating from the cell wall peptidoglycan of the Brevibacterium divaricatum. It is apyrogenic and non-toxic substance which exhibits adjuvant activity with different antigens2. The aim of this work was to determine immunogenicity in mice of five chimeric peptides comprising B cell epitope from MV hemagglutinin and different immunodominant T cell epitopes from MV fusion protein or nucleoprotein. As such, they represent immunogens with potential to elicit protective and neutralising antibodies. They differ in length (30-45 aa) depending whether they have one or two copies of either T cell or B cell epitope. We were interested whether the immune response to those peptides could be enhanced by addition of PGM as an adjuvant. Mice were immunised subcoutaneusly in the tail base with 50 mg or 100 mg of individual peptides, alone or in combination with 200 mg of PGM. Boosts were twice performed with the same dose in two week intervals. Peptides- or B cell epitope-specific antibodies were measured by the enzyme-linked immunosorbent assay (ELISA) established in laboratory. After immunisation we showed that anti-peptide antibody responses were different for each peptide. PGM increased the level of specific antibodies of two out of five tested peptides. All immunised mice sera exhibited strong crossreactivity with all tested chimeric peptides and B cell epitope peptide. However, none of the sera induced could neutralise measles virus (Edmonston Zagreb vaccine strain) in vitro. Finally, mice were immunised with a mixture (equal amounts) of the five chimeric peptides (50 mg and 250 mg total peptides). Specific antibody levels were greatly increased in comparison to levels obtained after single peptides immunisation, at the same concentration. Addition of PGM to such mixtures did not enhance specific antibodies production in mice. Further studies on the structure-activity relationship with respect to the aminoacid sequence of tested peptides and induced immune response are required. 1Karim C. El Kasmi, Dietmar Theisen, Nicolaas H.C. Brons, Wim Ammerlaan, Matthias Klingele, Anh T. Truong, Claude P. Muller: Vaccine 17 (1999) 2436-2445 2Jelka Tomašić, Ivana Hanzl-Dujmović, Beata Špoljar, Branka Vranešić, Maja Šantak, Aleksandra Jovičić: Vaccine 18 (2000) 1236-1243

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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