Naslov
Peptidoglycan monomer and its metabolic products - study of immunostimulating activity

Autori
Halassy Špoljar, Beata ; Krstanović, Marina ; Frkanec, Ruža ; Tomašić, Jelka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2001 Annual Meeting of the Croatian Immunological Society; Programme, Abstracts / - Zagreb : Hrvatsko imunološko društvo, 2001, 6-6

Skup
2001 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Zagreb, Hrvatska, 07.12.2001

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Sažetak
Peptidoglycan monomer, GlcNAc-MurNAc-L-Ala-D-isoGln-mesoDAP(wNH2) -D-Ala-D-Ala (PGM, PLIVA, Croatia) is a non-toxic, apyrogenic and water-soluble natural compound of bacterial origin. Recently we have shown using ovalbumin (OVA) as an antigen that PGM possesses adjuvant properties in mice model1 stimulating specific IgGs and IgAs in the sera and not affecting either total or specific IgEs. Subtype analysis of specific IgGs revealed that PGM stimulated both IgG1 as well as IgG2a and IgG2b classes of antibodies. Analysis of IFN-g and IL-4 production from OVA specific cells isolated from draining lymph nodes of immunised mice, revealed stimulative action of PGM on production of both cytokines, indicating that PGM is stimulator of both Th1 and Th2 types of immune response. Here we report on two aspects of PGM's action: its influence on induction of long lasting specific memory and the testing of possible adjuvant action of its metabolic products. It is known that PGM is the substrate for the amidase present in mammalian blood that cleaves it into metabolic products, the pentapeptide and the dissacharide2. For monitoring PGM's action on induction of long lasting specific memory mice were immunised twice in two week interval either solely with OVA or with OVA plus PGM. Six months later mice were immunised with antigen alone and the group that received PGM had higher level of OVA specific antibodies as measured by ELISA. Metabolic products of PGM, the pentapeptide and the disaccharide, were enzymatically prepared in vitro, separated and purified by gel filtration methods and their purity checked by high pressure liquid chromatography and thin layer chromatography. They were apyrogenic (250mg/kg) as tested by pyrogenicity test in rabbits. Their possible adjuvant effect was tested in parallel with PGM in mice model using OVA as an antigen. They were both devoid of adjuvant activity of their parent compound in this model. In conclusion, results obtained indicate that PGM is a molecule capable of stimulating long lasting specific immunity, whose action could possibly be improved in formulations that would slow down its degradation. 1Tomašić et al. (2000) Vaccine 18: 1236 2 Valinger et al. (1982) Biochym. Biophys. Acta 710:63

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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