Micronucleus, cell-free DNA and plasma glycan composition in the newborns of healthy and diabetic mothers

Fučić, Aleksandra; Guszak, Vedrana; Keser, Toma; Wagner, Jasenka; Juretić, Emilija; Plavec, Davor; Stojković, Ranko; Gornik, Olga; Lauc, Gordan

doi:10.1016/j.mrgentox.2017.01.002

Pregled bibliografske jedinice broj: 863839

Micronucleus, cell-free DNA and plasma glycan composition in the newborns of healthy and diabetic mothers

Fučić, Aleksandra; Guszak, Vedrana; Keser, Toma; Wagner, Jasenka; Juretić, Emilija; Plavec, Davor; Stojković, Ranko; Gornik, Olga; Lauc, Gordan

Micronucleus, cell-free DNA and plasma glycan composition in the newborns of healthy and diabetic mothers // Mutation research. Genetic toxicology and environmental mutagenesis, 815 (2017), 6-15 doi:10.1016/j.mrgentox.2017.01.002 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 863839 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Micronucleus, cell-free DNA and plasma glycan composition in the newborns of healthy and diabetic mothers

Autori
Fučić, Aleksandra ; Guszak, Vedrana ; Keser, Toma ; Wagner, Jasenka ; Juretić, Emilija ; Plavec, Davor ; Stojković, Ranko ; Gornik, Olga ; Lauc, Gordan

Izvornik
Mutation research. Genetic toxicology and environmental mutagenesis (1383-5718) 815 (2017); 6-15

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Diabetes ; Micronucleus assay ; Newborn ; Cell-free DNA ; Glycan

Sažetak
Diabetes is associated with certain environmental exposures, heritable factors, and metabolic conditions of intrauterine development due to diabetes in the mother. We evaluated genomic damage, cell-free DNA, N-glycosylation of umbilical cord plasma proteins (PG), and nuclear division index (NDI) as possible prognostic biomarkers of health risk in the newborns of mothers with treated pregestational diabetes(NBDM ; 22 mothers), compared these parameters with those from newborns of healthy mothers (NBHM ; 89 mothers), and associated the results with the mothers’ lifestyle in both groups, based on a detailed questionnaire. Genomic damage was estimated by the in vitro micronucleus (MN) assay. NDI was detectedon MN slides. Glycans were analyzed by ultra-performance liquid chromatography that separates theplasma N-glycome into 46 glycan peaks. Cell-free DNA was analyzed by real-time PCR. For the association between biomarkers and individual characteristics, generalized linear/nonlinear analysis was performed. No significant difference was found between NBHM and NBDM for cell-free DNA levels. There was no association between cell-free DNA levels and lifestyle. MN frequency was significantly higher in NBDM than in NBHM (median, 0.6 vs. 0.3%, p < 0.001). MN frequency and NDI were significantly associated withresidence (urban vs. rural). PG differed significantly between NBHM and NBDM (p < 0.001). A significant association was found between PG and increase of MN frequency (p < 0.001). As both MN frequency and altered N-glycosylation are associated with cancer risk, our study indicates need for further investigations.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb,
Medicinski fakultet, Osijek,
Dječja bolnica Srebrnjak,
GENOS d.o.o.

Profili:

Davor Plavec (autor)