Naslov
Cellular hydrogels based on pH-responsive chitosan- hydroxyapatite system

Autori
Rogina, Anamarija ; Ressler, Antonia ; Matić, Igor ; Gallego Ferrer, Gloria ; Marijanović, Inga ; Ivanković, Marica ; Ivanković, Hrvoje

Izvornik
Carbohydrate polymers (0144-8617) 166 (2017); 173-182

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Chitosan ; Hydroxyapatite ; pH-responsive hydrogel ; Rheology ; Cell encapsulation
(chitosan ; hydroxyapatite ; pH-responsive hydrogel ; rheology ; cell encapsulation)

Sažetak
The development of bioactive injectable system as cell carrier with minimal impact on viability of encapsulated cells represents a great challenge. In the present work, we propose a new pH- responsive chitosan-hydroxyapatite-based hydrogel with sodium bicarbonate (NaHCO3) as the gelling agent. The in situ synthesis of hydroxyapatite phase has resulted in stable composite suspension and final homogeneous hydrogel. The application of sodium bicarbonate has allowed non-cytotoxic fast gelation of chitosan-hydroxyapatite within 4 minutes, and without excess of sodium ions concentration. Rheological properties of crosslinked hydrogel have demonstrated possible behaviour as ‘strong physical hydrogel’. The live dead staining has confirmed good viability and dispersion, as well as proliferation of encapsulated cells by the culture time. Presented preliminary results show good potential of chitosan-hydroxyapatite/NaHCO3 as a cell carrier, whose impact on the cell differentiation need to be confirmed by encapsulation of other cell phenotypes.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Kemijsko inženjerstvo, Temeljne tehničke znanosti

Napomena
Glavni autori: Anamarija Rogina i Marica Ivanković

POVEZANOST RADA