Naslov
Non-toxic fluorescent phosphonium probes to detect mitochondrial potential

Autori
Šarić, Ana ; Crnolatac, Ivo ; Bouillaud, Frédéric ; Sobocanec, Sandra ; Mikecin, Ana- Matea ; Mačak Šafranko, Željka ; Deligeorgiev, Todor ; Piantanida, Ivo ; Balog, Tihomir ; Petit, Patrice

Izvornik
Methods and applications in fluorescence (2050-6120) 5 (2017), 1; 015007, 13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Fluorescence ; Mitochondria ; Membrane potential ; Probe ; Phophonium

Sažetak
We evaluated our phosphonium-based fluorescent probes for selective staining of mitochondria. Currently used probes for monitoring mitochondrial membrane potential show varying degrees of interference with cell metabolism, photo-induced damage, and probe binding. Here presented probes are characterized by highly efficient cellular uptake and specific accumulation in mitochondria. Fluorescent detection of the probes was accomplished using flow cytometry and confocal microscopy imaging of yeast and mammalian cells. Toxicity analysis (impedimetry - xCELLigence for the cellular proliferation and Seahorse technology for respiratory properties) confirms that these dyes exhibit no toxicity on mitochondrial or cellular functioning even for long time incubation. The excellent chemical and photophysical stability of the dyes make them promising leads toward improved fluorescent probes. Therefore, the probes described here offer to circumvent the problems associated with existing probes limitations.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA