Naslov
Chemical Composition and Biological Activity of Allium cepa L. and Allium x cornutum (Clementi ex Visiani 1842) Methanolic Extracts

Autori
Fredotović, Željana ; Šprung, Matilda ; Soldo, Barbara ; Ljubenkov, Ivica ; Budić-Leto, Irena ; Bilušić, Tea ; Čikeš-Čulić, Vedrana ; Puizina, Jasna

Izvornik
Molecules (1420-3049) 22 (2017), 3; 448, 13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
onions ; A. × cornutum ; A. cepa ; phenolic compounds ; antioxidant activity ; genotoxicity ; antimutagenic activity ; antiproliferative activity

Sažetak
Here we report a comparative study of the pyhtochemical profile and a biological activity of the two onion extracts, namely A. cepa L. and A. × cornutum (Clementi ex Visiani 1842). Identification of flavonoids and anthocyanins and their individual quantities were determined by high performance liquid chromatography (HPLC). The potency of both extracts to scavenge free radicals was determined by DPPH (2, 2'-diphenyl- 1- picrylhydrazyl) radical- scavenging activity and oxygen radical absorbance capacity (ORAC) methods. DNA protective role was further tested by single- cell gel electrophoresis (COMET) assay and by Fenton’s reagent causing double strand brakes on pUC19 plasmid. In the presence of both extracts, a significant decrease in the DNA damage was observed, which indicates a protective role of A. cepa and A. × cornutum on the DNA strand brakes. Additionally, cytotoxicity was tested on glioblastoma and breast cancer cell lines. The results showed that both extracts had antiproliferative effect, but the most prominent decrease in cellular growth was observed with glioblastoma cells

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Prehrambena tehnologija

POVEZANOST RADA