Naslov
Microwave-Assisted Extarction (MAE) of Dalmatian Sage Leaves for the Optimal Yield of Polyphenols : HPLC_DAD Identification and Quantification

Autori
Putnik, Predrag ; Bursać Kovačević, Danijela ; Penić, Marija ; Fegeš, Maja ; Dragović-Uzelac, Verica

Izvornik
Food Analytical Methods (1936-9751) 9 (2016), 8; 2385-2394

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Microwave-Assisted Extarction, Salvia officinalis L., Dalmatian sage, Phenolic acid, Solvent, Temperature

Sažetak
Dalmatian sage (Salvia officinalis L.) is a Croatian medicinal herb with important biological and pharmacological properties due to diverse polyphenolic composition. Therefore, this study aimed at evaluating the effects of three solvents (30 % ethanol/water, v/v ; 30 % acetone/water, v/v ; water), time (3, 5, 7, 9, and 10 min), temperature (30, 50, 60, and 80 °C), and acidity (addition of 10 % HCl) with advanced extraction techniques such as microwave-assisted extraction (MAE) on phenolic compounds in Dalmatian sage extracts. Total polyphenols were better extracted with 30 % aqueous media with acetone (v/v), without HCl at 80 °C for 10 min. Considering individual phenolic compounds, in all extracts, rosmarinic acid was predominant and better extracted with 30 % ethanol/added HCl at 80 °C. The optimal polyphenol extraction conditions were methyl rosmarinate (30 % ethanol/added HCl/32 °C), syringic acid (30 % ethanol/ without HCl/80 °C), salvianolic acid (any solvent/without HCl/80 °C), caffeic acid (water/added HCl/80 °C), and vanillic acid (30 % ethanol/added HCl/80 °C).

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

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