Naslov
The triparental triploid onion Allium x cornutum (Clementi ex Visiani, 1842) possesses a sterile S-type of cytoplasm

Autori
Fredotović, Željana ; Šamanić, Ivica ; Kamenjarin, Juraj ; Puizina, Jasna

Izvornik
Genetic resources and crop evolution (0925-9864) 64 (2017), 8; 1971-1983

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Allium ; chloroplast DNA ; cytoplasmic male-sterility ; genomic in situ hybridization ; Ljutika ; Pran ; triploid onions

Sažetak
Triploid onion, Allium × cornutum (Clementi ex Visiani, 1842) (2n = 3x = 24), a vegetatively reproduced garden crop, possess a complex triparental genome organization with three putative parental species, A. cepa L., A. pskemense B. Fedtsh., and A. roylei Stearn. Two of its most studied clones are the Croatian 'Ljutika' and the Indian 'Pran', which are genetically highly similar. Earlier studies have shown that 'Pran' possesses some molecular markers in the chloroplast DNA (cpDNA) identical to those of the unique male-sterile (S) cytoplasm, used for onion breeding. To find out whether 'Ljutika' also possesses a S-type of cytoplasm, we analyzed several cpDNA and mitochondrial (mtDNA) molecular markers. The PCR amplification and RFLP analysis of the chloroplast genes accD, atpF, petB and the mitochondrial gene cob, as well as the sequence analysis of the chloroplast matK and atpB-rbcL regions showed that 'Ljutika' possesses the male- sterile S- type of cytoplasm. The phylogenetic analysis of the matK and atpB-rbcL sequences of A. ×cornutum, its parental species and other Allium species of the section Cepa, showed that none of the analyzed species had the identical type of cpDNA as A. × cornutum. Results also suggested that A. pskemense can be excluded as a donor of the S-cytoplasm and a female parent, whereas cpDNA of A. roylei, although not identical to S- cytoplasm, possessed many polymorphisms of S-type. Fluorescence in situ hybridization, using fluorescently labelled parental genomic DNAs as probes in combination with fluorescently labelled 5S and 35S rDNAs enabled simultaneous visualization of the three genomes during meiosis and confirmed their homeologus intergenomic pairing.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija

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