Naslov
A concomitant reciprocal expression of p53 and beta-catenin in meningiomas

Autori
Kafka, Anja ; Babić Leko, Mirjana ; Njirić, Niko ; Logara, Monika ; Mrak, Goran ; Pećina-Šlaus, Nives

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EACR Conference: A matter of life or death / - , 2016

Skup
EACR Conference: A matter of life or death

Mjesto i datum
Amsterdam, Nizozemska, 28.01.2016. - 30.01.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
p53 ; beta-catenin ; meningioma

Sažetak
Cellular expression of p53 and beta-catenin proteins, and genetic changes of TP53 in 59 meningiomas were analyzed by immunohistochemistry and PCR/RFLP. Gross deletion of p53 gene was observed in one meningioma (9% of informative samples). Our analyses showed that 47, 5% of our total sample demonstrated loss of expression of p53 protein. Moderate and strong p53 expression was noted mostly in nucleus in 8, 5% and 6, 8% of meningiomas respectively. Contrary, beta- catenin, the main Wnt signaling molecule was upregulated in 71, 2% and strong expression was observed in 28, 8% of meningiomas. The levels of the two proteins were significantly strongly negatively correlated in the analyzed meningiomas (P=0, 002). This indicates that meningiomas with lost p53 upregulate beta- catenin and activate Wnt signaling. Besides showing the reciprocal relationship between beta-catenin and p53, we also showed that the expression of p53 was significantly (P=0, 021) associated to higher meningioma grades (II and III) while beta-catenin's upregulation was not associated to malignancy grades. Furthermore, women exhibited significantly higher values of p53 loss when compared to males (P=0, 005). The ongoing research is aiming to offer new molecular markers for meningiomas based on the genetic pathways and molecules involved and help to develop new treatment modalities.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA