Naslov
Kinetic Spectrophotometric Determination of N-acetylcysteine ethyl Ester using Bichinchoninic Acid

Autori
Kukoč-Modun, Lea ; Tsikas, Dimitrios ; Kraljević, Tomislav ; Biočić, Maja ; Radić, Njegomir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Bulletin of the Chemists and Technologists of Bosnia and Herzegovina / Korać, Fehim - Sarajevo : Prirodno-matematički fakultet Sarajevo, 2016, 51-51

Skup
2nd International Congress of Chemists and Chemical Engineers of Bosnia and Herzegovina

Mjesto i datum
Sarajevo, Bosna i Hercegovina, 21.10.2016. - 23.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
N-Acetylcysteine ethyl ester; Bicinchoninic acid; Kinetic spectrophotometry; Fixed time method.

Sažetak
N-acetylcysteine ethyl ester (NACET) according to reported data has the potential to substitute NAC as a mucolytic agent, as a paracetamol antidote and as a glutathione related antioxidant. NACET once it is administered quickly enters the cells where it is transformed to NAC and cysteine. A novel kinetic spectrophotometric method of analysis is developed for the determination of NACET with the use of Bicinchoninic acid (2-(4-Carboxyquinolin-2-yl)quinoline-4-carboxylic acid, BCA), a highly sensitive and specific reagent for Cu+-ions. The proposed method is based on a reduction reaction of a Cu(BCA)22+ complex by NACET to a purple Cu(BCA)2+ complex in a phosphate buffer solution. The absorbance of the generated complex is measured at 562 nm. The parameters optimized included: pH of the reaction solution, the effect of temperature, the molar ratio of Cu2+/NACET, the molar ratio of BCA/NACET and the molar ratio of Cu2+/BCA. The effect of interfering substances was also investigated. A fixed time method was utilized for the construction of a linear calibration graph from 4.0×10–7 to 1.0×10–4 mol L–1 of NACET by the use of the kinetic spectrophotometric method where the detection limit was 1.6×10–7 mol L–1.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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