Naslov
Total lipid extraction from archaeological samples from Ancient sites on the island of Brač

Autori
Gluščić, Valentina ; Horvatiček, Marina ; Jelinčić Vučković, Kristina ; Delaš, Ivančica.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Methodology&Archaeometry, 04th scientific conference / Miloglav, Ina. - Zagreb, 2016, 36-36

ISBN
978-953-6335-10-7

Skup
04th Scientific Conference Methodology and Archaeometry

Mjesto i datum
Zagreb, Hrvatska, 01.12.2016. - 02.12.2016

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
total lipid extraction; amphorae; Tripolitana II; Tripolitana III; olive oil

Sažetak
During archaeological survey of Ancient sites on the island of Brač, fragments of different amphorae types were found. Preliminary results of amphorae samples analyses are presented, types Tripolitana II, Tripolitana III, Tripolitana III (late) and Keay 59/Bonifay 37 from sites of Njivice Selca, Luke Škrip, Mladinje Brdo Pučišća and Bunje Novo Selo. Typological analyzes and bibliography suggests that found amphorae were used for oil transport and/or store. Due to these facts in this preliminary research we determine the composition of total lipids and wanted to confirm the function of these amphorae as olive oil containers. Samples of different types of amphorae where analysed: V3 (Tripolitana II, site: Njivice Selca), V4 (Tripolitana III, site: Luke V5 (Tripolitana III, (late), site: Mladinje Brdo Pučišća) and V13 (type of amphora: Keay 59/Bonifay 37, site: Bunje Novo Selo). They were taken from necks or inner lip side. Traces of adsorbed lipids resin were present in all sample extracts. The extractions of Total lipids from 4 samples were extracted according to method described by Mottram et all. (1999). The extraction was performed with solvent mixtures of different polarity, obtained by combining chloroform and methanol in different ratios. The analysis of fatty acid methyl esters (FAME) composition was performed on gas chromatograph with flame ionization detector. In two extracts of Amphora sample type Tripolitana III qualitative analysis was performed and compounds of fatty acids were identified. These types of amphora were used for storage of vegetable olive oil. In these samples we have confirmed the presence of the following fatty acids: C10:0, C11:0, C13:0, C15:0, C16:0, C16:1, C18:0, C18:2, C18:3, C20:0, C20:5, C24:1. A chromatogram of the sample Tripolitana II identified small abundance of fatty acids: C20:0, C21:0, C20:4. In extract of the sample Keay 59/ Bonifay 37 it was identified the presence of: C10:0, C11:0, C15:0, C15:1, C16:0, C18:0, C20:0, C20:4, C20:5, C24:1. It is also known that this type of amphora was used for storage and transport of oil. The presence of oleic fatty acid, C18:1, as biomarker for olive oil residues in our samples was not confirmed. However, this compound, as well as other mono-and polyunsaturated fatty acids are prone to oxidation processes and are poorly preserved in archaeological samples. Porous amphorae material adsorbs lipids into the matrix and reduces the exposure to oxygen, air, sunlight and heat that cause decomposition process. Palmitic (C16:0) and stearic (C18:0) fatty acids are present in all samples in higher abundance. Presence of long chain fatty acids such as C20:0, C21:0, C22:0 and C24:0 could be related to the presence of waxes. The presence of C22:0 also indicates the presence of plant oils in residues. Due to small abundances of fatty acids in our samples we cannot confirm with certainty the presence of olive oil in these amphorae. Therefore in further research and analysis of archaeological samples different sample preparation methods could be applied.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Arheologija

POVEZANOST RADA