Naslov
Micronucleus, cell free DNA and glycan levels in newborns of healthy and diabetic mothers

Autori
Fučić, Aleksandra ; Guzsak, Vedrana ; Keser, Toma ; Wagner, Jasenka ; Juretić, Emilija ; Plavec, Davor ; Chalkiadaki, Georgia ; Stojković, Ranko ; Gornik, Olga ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EEMGS Anual Meeting Programme & Abstract book / - Kopenhagen : University of Copenhagen, 2016, 64-64

Skup
EEMGS Annual Meeting

Mjesto i datum
Kopenhagen, Danska, 14.08.2016. - 18.08.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
diabetes; micronucleus assay; newborn; free DNA; glycan

Sažetak
Diabetes is described to be associated with some types of environmental exposure, heredtital factors and with specific metabolic conditions of intrauterine development due to diabetes in the mother. The aim of the present study was to evaluate genome damage, cell free DNA, protein glycosylation (PG) and nuclear division index (NDI) as possible prognostic biomarkers for health risks in newborns of mothers with treated pregestational diabetes (NBDM), compare them with newborns of healthy mothers (NBHM), and to associate results with mother lifestyle in both groups using detailed questionanire. 89 NHM and 22 NDM were included in study. Genome damage was estimated by in vitro micronucleus (MN) assay. NDI was detected on MN slides. Glycans were analysed by ultra- performance liquid chromatography that separates plasma glycome into 46 glycan peaks. Cell free DNA was analysed by real time PCR. For the association between biomarkers and individual characteristics stepwise logistic analysis was performed. No significant difference was found between NHM and NDM for cell free DNA levels (mean, 0.003 vs. 0.001 µg/ml, p=0.114). There was no association between cell free DNA level with lifestyle. MN frequency was significantly higher in NDM than in NHM (median, 0.6 vs. 0.3 %, p<0.001). MN frequency and NDI were significantly associated with residence (urban vs. agrar). PG significantly differed between NHM and NDM. A significant association was was found between PG and increase of MN frequency. As both, MN frequency and altered PG are associated to cancer risk, our study urges for further investigations.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti

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