Naslov
Labelling neural stem cells with different superparamagnetic iron oxide nanoparticles results in change of membrane potential and DNA damage

Autori
Pongrac, Igor M. ; Pavičić, Ivan ; Milić, Mirta ; Brkić Ahmed, Lada ; Babic, Michal ; Mlinarić, Hrvoje ; Horák, Daniel ; Mitrečić, Dinko ; Vinković Vrček, Ivana ; Gajovic ; Srećko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology - HDBMB2016 on the occasion of the 40th Anniversary / - Split, 2016, 96-96

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology - HDBMB2016 on the occasion of the 40th Anniversary

Mjesto i datum
Split, Hrvatska, 01.06.2016. - 04.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
labelling ; neural stem cells ; superparamagnetic iron oxide nanoparticles ; membrane potential ; DNA damage

Sažetak
INTRODUCTION: Biocompatibility, safety and risk assessments of superparamagnetic iron oxide nanoparticles (SPIONs) are of the highest priority in testing their potential applications in biomedicine. Improvement of the biological properties of SPIONs may be achieved by different functionalization and surface modifications. AIMS: This study investigated how different surface functionalizations of SPIONs – uncoated, coated with D-mannose or coated with poly-L-lysine - affected biocompatibility. By using murine neural stem cells (NSCs) as important model system for the regenerative medicine we had a goal to reveal the possible mechanism of toxicity of SPIONs on NSCs, mitochondrial membrane potential, cell membrane potential and DNA damage. RESULTS: We observed hyperpolarization of mitochondrial membrane, dissipated cell membrane potential and increased DNA damages in NSCs, irrespective of the surface coating applied for SPION stabilization. Although surface coating should prevent the toxic effects of SPIONs, our results showed that all of the tested SPION types affected the NSCs similarly indicating that mitochondrial homeostasis is their major cellular target. CONCLUSIONS: Despite the claimed biomedical benefits of SPIONs, the refined determination of their effects on various cellular functions presented in this work highlights the need for further safety evaluations. This investigation helps to fill the knowledge gaps on the criteria that should be considered in evaluating the biocompatibility and safety of novel nanoparticles. ACKNOWLEDGMENTS: This study was supported by EU FP7 grant GlowBrain (REGPOT-2012-CT2012-316120), by EU ESF project MladiMozak (030201-0180) and by the Czech Science Foundation (project no. 16-01128J).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA