ࡱ> EHDD<bjbj4B<N:0b(Y[[[[[[$j  #<u111Y1Y1111xA.1E 0:1H#1H#11hH#11:H# :Utvrivanje fizioloakog broja somatskih stanica u ov jem mlijeku Iva Dolen i `pehar 1*, `imun Zamberlin 1, Nikolina Kelava Ugarkovi 2, Dubravka Samar~ija 1 1Sveu iliate u Zagrebu, Agronomski fakultet, Zavod za mljekarstvo, Svetoaimunska 25, 10000 Zagreb, Hrvatska 2Sveu iliate u Zagrebu, Agronomski fakultet, Zavod za specijalno sto arstvo, Svetoaimunska 25, 10000 Zagreb, Hrvatska *Dopisni autor: HYPERLINK "mailto:ispehar@agr.hr"ispehar@agr.hr Sa~etak Fizioloaki broj somatskih stanica (FBSS) u ov jem mlijeku joa nije utvren. Prema podatcima iz literature vime se smatra zdravim ako FBSS ne prelazi 500 000 stanica/mL a omjer izmeu limfocita (LF), makrofaga (MF) i polimorfonuklearnih leukocita (PMN) odreen je pasminom, periodom i brojem laktacije. Cilj ovog istra~ivanja bio je utvrditi FBSS u mlijeku dalmatinske pramenke i isto nofrizijske ovce ije se mlijeko u Hrvatskoj naj eae koristi za proizvodnju sira. Zbog toga je za kvalitetu sira utvrivanje FBSS u ov jem mlijeku od posebnog zna enja. Ukupno je prikupljeno i analizirano 60 uzoraka mlijeka dalmatinske pramenke i 105 uzoraka mlijeka isto nofrizijske ovce tijekom 1., 2., i 3. laktacije. Nakon utvrivanja broja somatskih stanica (BSS), za utvrivanje omjera izmeu LF:MF:PMN uzorci mlijeka podijeljeni su dvije skupine: I. d" 200 000 somatskih stanica/mL i II. od 200 do 500 000 somatskih stanica/mL. Prosje an BSS u mlijeku dalmatinske pramenke u 1. laktaciji bio je 79 000 stanica/mL, u 2. 87 800 stanica/mL, a u 3. 70 900 stanica/mL, a omjer LF: MF : PMN za BSS d"200 000 /mL bio je 65:25:9. U mlijeku isto nofrizijske ovce prosje an BSS u 1. laktaciji bio je 107 000 stanica/mL, u 2. 112 000 stanica/mL, a u 3. 105 000 stanica/mL, a omjer LF: MF : PMN za BSS d"200 000 /mL bio je 59:17:11. Pasmina ovaca imala je zna ajan utjecaj (p<0,01) na FBSS u prve tri laktacije. Neovisno o pasmini, broju i periodu laktacije promjene LF:MF:PMN nisu utvrene za BSSd" 200 000 /mL. Za obje pasmine utvreno je zna ajno (p<0,0001) poveanje PMN unutar stanica leukocita za mlijeko u kojem je BSS bio od 200 do 500 000/mL. Prema dobivenim podatcima FBSS tijekom 1., 2. i 3. laktacije za dalmatinsku pramenku i isto nofrizijsku pasminu ovaca vjerojatno je izmeu 100 000 i 250 000 stanica/mL. Klju ne rije i: fizioloaki broj somatskih stanica, ov je mlijeko, limfociti, makrofagi, polimorfonuklearni leukociti Determination of physiological somatic cell count in sheep milk Iva Dolen i `pehar 1*, `imun Zamberlin 1, Nikolina Kelava Ugarkovi 2, Dubravka Samar~ija 1 1University of Zagreb, Faculty of Agriculture, Department of Dairy Science, Svetoimunska 25, 10 000 Zagreb, Croatia 2University of Zagreb, Faculty of Agriculture, Department of Animal Science and Technology, Svetoimunska 25, 10 000 Zagreb, Croatia * Corresponding author: HYPERLINK "mailto:ispehar@agr.hr"ispehar@agr.hr Abstract Physiological somatic cell count (PSCC) in sheep's milk has not been determined. According to literature data, the udder is considered to be healthy if PSCC  : @ B   $ & h j  LNDFHJ`̾yyyhUhrx\hUhT\hUhsc\hUh+\hUhR\ hUhR hUh] hUhschUhr5hUhr0J6hgjhgUhUhr6hUhr6H*hUhrH* hUhrhUhrCJaJ/ > @   24gdr$a$gdU $dha$gdr&J,.0468xt| 8TX068>flt~÷﷯篣÷﷯篊hUh}m\ hU\hUh-`s\mH$sH$hUh2\mH$sH$hUh>\hUh>\mH$sH$hUh^>\mH$sH$hUhrx\hUhX?\hUh2\hUhsc\hUh^>\hUhR\5LNT~@J48:HVDFL|ӻӯӋӻwwiahUhr5hUhr5B* \phphUhrx\mH$sH$hUh\hUh\mH$sH$hUh|%\mH$sH$hUhr \mH$sH$hUhX?\mH$sH$hUhrx\hUh+\hUhsc\hUh}m\hUhn\mH$sH$hUhT\mH$sH$hUh}m\mH$sH$#2\`mn   -.ʼqbTLHLhgjhgUhUhX?6\mH sH hUhX?mH nH sH tH "hUhX?56CJOJQJaJ*hUhX?56CJOJQJaJmH sH -hUhX?56CJH*OJQJaJmH sH hUhX?6mH sH hUhX?6H*mH sH hUhX?H*hUhX?CJaJ hUhX? hUh, hUh5} hUhr hUh2m>?@AJP;R;;:<<<><@<B<D< $dha$gd N $dha$gdU$a$gdU$a$gdU $ $a$gdU.<=>AJOo,.-:-l-----b.h....*/2////H0H1x11 2(20222<2R2d2r22ʿ~s~~hhmH sH hh{dmH sH hhUmH sH hhGmH sH hh VmH sH hh NmH sH UhmH sH hhX?mH sH hUhX?5mH sH  hUhX?hUhX?6mH sH jhgUhUhX?0J6mH sH ( not exceed 500 000 cells / ml and the ratio of the lymphocytes (LF), macrophages (MF) and polymorphonuclear leukocytes (PMN) was determined by breed, number and stage of lactation. The aim of this research was to determine PSCC in milk of Dalmatian Pramenka and East Friesian sheep whose milk in Croatia is most frequently used for cheese production. Therefore, for the quality of cheese determination of PSCC in sheep's milk is extremely important. In total, 60 milk samples of Dalmatian Pramenka and 105 milk samples of the East Friesian sheep were collected and analyzed during the 1st, 2nd, and 3rd lactation. Fluoro-opto-electronic method was used for determination of somatic cell count (SCC) and microscopic method for the differentiation of somatic cells. After determination of SCC, for obtaining the ratio between LF: MF: PMN, milk samples were divided into two groups: I. d" 200 000 somatic cells/ml and II. from 200 to 500 000 somatic cells/ml. The average milk SCC of Dalmatian Pramenka on the 1st lactation was 79 000 cells/ml, the 2nd 87 800 cells/ml, and in 3rd 70 900 cells / ml, and the ratio of LF: MF: PMN for the SCC d"200 000/ml was 65: 25: 9. The average milk SCC of East Friesian sheep in the 1st lactation was 107 000 cells / ml, the 2nd 112 000 cells / ml, and the 3rd 105 000 cells / ml, and the ratio of LF: MF: PMN for the SCC d"200 000/ml was 59:17:11. The sheep breed had a significant influence (p <0.01) on the PSCC in the first three lactations. Regardless of the breed, the number and the period of lactation, changes of the ratio LF: MF: PMN were not established for the SCCd" 200 000/ml. For both breeds a significant (p <0.0001) increases were determined for PMN within the leukocyte cells in milk containing SCC from 200 to 500 000/ml. According to the results of PSCC in milk during the 1st, 2nd and 3rd lactation for the Dalmatian Pramenka and East Friesian sheep breed are probably between 100 000 and 250 000 cells/ml. Keywords: somatic cells, sheep's milk, lymphocytes, macrophages, polymorphonuclear leukocytes 2203:333333P44445\5^566666666F7L7N7j8888\9p9|9999 ::::: ::::$;*;H;L;N;P;R;d;<꣗hl9>hUmH sH hUhU5mH sH hUB*ph"""hhmH sH hhUmH sH hh VmH sH hhGmH sH hhr mH sH hmH sH hh{dmH sH hh mH sH 6< <:<<<@<B<D< hUhX?hUhX?mH sH hUh NmH sH  hUhUhl9>hUmH sH hUmH sH 21h:pX?. 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