Naslov
Alterations in Plasma Proteome of Dogs with Obesity- Related Metabolic Dysfunction: Preliminary Results

Autori
Asta Tvarijonaviciute ; Carlos de Torre ; Blanka Beer Ljubić ; Shelley L. Holden ; Vincent Biourge ; Penelope J. Morris ; Josep Pastor ; Jose J. Ceron ; Alexander J. German

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
25th Congress of the European College of Veterinary Internal Medicine - Companion animals

Mjesto i datum
Lisabon, Portugal, 10.09.2015. - 12.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Antioxidants; Complement system; Dog; Immune response; Lipid metabolism; Metabolic syndrome; Obesity

Sažetak
In humans, the metabolic syndrome (MS) is a well- recognised and extensively studied entity that comprises obesity, hypertension, dyslipidaemia, and glucose intolerance. It is associated with an increased risk of cardiovascular diseases and diabetes. Recently, human MS criteria were adapted for dogs to define the condition of obesity-related metabolic dysfunction (ORMD). It was observed that ORMD was associated with increased circulating insulin and decreased adiponectin concentrations, suggesting that in dogs, as in humans, there are links between obesity, ORMD, and associated diseases, although pathogenetic mechanisms and health significance for dogs remain unknown. The main aim of the present study was to compare plasma proteomes of obese dogs with and without ORMD, so as to investigate the mechanisms associated with canine ORMD and their possible significance in the health status. Eight obese dogs referred for weight management at the Royal Canin Weight Management Clinic, University of Liverpool, participated in the study. Clinical assessments included physical examination, body condition scoring, blood pressure measurement and routine clinicopathological analysis. Surplus plasma was used in proteomic analysis. Samples were first treated with ProteoMiner for the depletion of high-abundance proteins and subsequently analysed by using 2-DE DIGE methodology. Of the eight dogs in the study, 4 dogs had ORMD and 4 dogs did not. Image analysis and further statistical analysis allowed identification of 8 spots with differential expression concentration between dogs with and without ORMD. Among the 8 spots, 3 were overexpressed and 5 were down- expressed in dogs with ORMD than in dogs that did not present with ORMD. Although the results of the present study are preliminary and still the identification of the spots is up to be performed, the observed data reveals that dogs with ORMD present alterations in their plasma proteomes that could be responsible for the development of ORMD-related pathologies.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA