Pregled bibliografske jedinice broj: 842333
Inflammatory monocytes and NK cells play a crucial role in DNAM-1-dependent control of cytomegalovirus infection
Inflammatory monocytes and NK cells play a crucial role in DNAM-1-dependent control of cytomegalovirus infection // Journal of experimental nanoscience, 213 (2016), 9; 1835-1850 doi:10.1084/jem.20151899 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 842333 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Inflammatory monocytes and NK cells play a crucial role in DNAM-1-dependent control of cytomegalovirus infection
Autori
Lenac Roviš, Tihana ; Kučan Brlić, Paola ; Kaynan, N. ; Juranić Lisnić, Vanda ; Brizić, Ilija ; Jordan, S. ; Tomić, Adriana ; Kveštak, Daria ; Babić, Marina ; Tsukerman, P. ; Colonna, M. ; Koszinowski, U. ; Messerle, M. ; Mandelboim, O. ; Krmpotić, Astrid ; Jonjić, Stipan
Izvornik
Journal of experimental nanoscience (1745-8080) 213
(2016), 9;
1835-1850
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
MCMV ; evasion ; immune control ; DNAM-1 ; PVR ; NK cell ; monocyte
Sažetak
The poliovirus receptor (PVR) is a ubiquitously expressed glycoprotein involved in cellular adhesion and immune response. It engages the activating receptor DNAX accessory molecule (DNAM)-1, the inhibitory receptor TIGIT, and the CD96 receptor with both activating and inhibitory functions. Human cytomegalovirus (HCMV) down-regulates PVR expression, but the significance of this viral function in vivo remains unknown. Here, we demonstrate that mouse CMV (MCMV) also down-regulates the surface PVR. The m20.1 protein of MCMV retains PVR in the endoplasmic reticulum and promotes its degradation. A MCMV mutant lacking the PVR inhibitor was attenuated in normal mice but not in mice lacking DNAM-1. This attenuation was partially reversed by NK cell depletion, whereas the simultaneous depletion of mononuclear phagocytes abolished the virus control. This effect was associated with the increased expression of DNAM-1, whereas TIGIT and CD96 were absent on these cells. An increased level of proinflammatory cytokines in sera of mice infected with the virus lacking the m20.1 and an increased production of iNOS by inflammatory monocytes was observed. Blocking of CCL2 or the inhibition of iNOS significantly increased titer of the virus lacking m20.1. In this study, we have demonstrated that inflammatory monocytes, together with NK cells, are essential in the early control of CMV through the DNAM-1–PVR pathway
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
062-0621261-1263 - Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru (Jonjić, Stipan, MZOS ) ( CroRIS)
062-0621261-1268 - Uloga imunosubverzivnih citomegalovirusnih gena u latenciji (Krmpotić, Astrid, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Daria Kveštak
(autor)
Astrid Krmpotić
(autor)
Ilija Brizić
(autor)
Vanda Juranić Lisnić
(autor)
Adriana Tomić
(autor)
Stipan Jonjić
(autor)
Paola Kučan Brlić
(autor)
Tihana Lenac Roviš
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus