Naslov
Genotoxic effects of glyphosate in human derived hepatoma (HepG2) cells assessed by cytome assay

Autori
Kašuba, Vilena ; Milić, Mirta ; Kopjar, Nevenka ; Mladinić, Marin ; Želježić, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 5th Croatian Congress of Toxicology with International Participation / Durgo, Ksenija - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2016, 44-44

Skup
5th Croatian Congress of Toxicology with International Participation

Mjesto i datum
Poreč, Hrvatska, 09.10.2016. - 12.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
cytome assay ; genotoxic effect ; glyphosate ; human derived hepatoma cells

Sažetak
Glyphosate is the most widely used nonselective herbicide. Until recently, it was considered environmentally safe and minimally toxic to humans. Recent studies suggest that glyphosate and its metabolites could affect normal human cell development. In this study, glyphosate was tested at concentrations of 0.5, 2.91, and 3.5 μg mL-1, which corresponded to the values of acceptable daily intake (ADI), residential exposure level (REL), and occupational exposure level (OEL). Analyses of micronuclei, nuclear buds, nucleoplasmic bridges, apoptosis/necrosis, and nuclear division index in HepG2 cells were performed following 4 and 24 hours of in vitro treatment. The results demonstrated that 4-hour exposure to glyphosate slightly increased cytogenetic damage in terms of micronuclei, statistically significant only at OEL. Despite a nonsignificant increase in micronuclei frequencies at ADI and REL, a significant increase in nuclear bud frequency was found. Since nuclear budding represents a micronuclei formation mechanism, our results indicate that even a low dose such as ADI can influence the DNA/cytogenetic damage level. After 24h of exposure, a lower number of binucleated cells was found at all of the three tested concentrations compared to control. Nuclear bud frequency changed only in the ADI sample, while in the OEL- and REL-treated samples, it was significantly lower. The control and treated cells did not significantly differ in the number of nucleoplasmic bridges. It is possible that the two exposure times selected in this study were too short for a reliable estimation of glyphosate genotoxicity, which has to be further clarified through other in vitro/ in vivo models. This work was financially supported by Project No.8366 Organic Pollutants in Environment – Markers and Biomarkers of Toxicity (OPENTOX), funded by the Croatian Science Foundation.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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