Naslov
Dose-dependent early recruitment of intestinal intraepithelial gama delta T cells following experimental Trichinella spiralis gut infection

Autori
Dominis Kramarić, Miroslava ; Božić, Frane ; Marinculić, Albert

Izvornik
Helminthologia (0440-6605) 37 (2000), 4; 187-193

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Trichinella spiralis; dose-dependent response; gama delta T cells; intraepithelial lymphocytes; gut

Sažetak
Since the expulsion of Trichinella spiralis worms is a dose-dependent effect, to assess whether or not intestinal intraepithelial lymphocytes (i-IELs), particularly T cells, respond comparably to different sized T. spiralis infection, we used C57BL mice, orally infected with two doses, 50 or 400 viable T. spiralis muscle larvae (L1). In the present study we found that the appearance of T cells preceded that of T cells among i-IELs of infected mice. The proportion of T cells increased promptly (on day 1 p.i.) and significantly (P 0.005) in the gut epithelium of mice infected with 400 L1 T. spiralis larvae, but not in mice infected with 50 L1 larvae, compared with uninfected mice. These data indicate that T cell responses varied in a dose-dependent manner. In spite of their rapid increase, no correlation between kinetics of nor T cells in i-IELs and T. spiralis worm expulsion was observed in mice infected with either 400 (because of a rapid decrease in the i-IEL population in gut epithelium early after activation, by day 7 p.i.) or 50 L1 larvae (since no statistically significant rise in the proportion of or T cells was observed during the course of infection). Alltogether, our results suggest that both T. spiralis infection levels probably elicit immunodepression by interference with the first line defence of T cell function thereby influencing the outcome of experimental T. spiralis gut infection.

Izvorni jezik
Engleski

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