Naslov
NF1 tumor supresor gene in colon cancer: loss of heterozygosity

Autori
Kapitanović, Sanja ; Čačev, Tamara ; Spaventi, Radan ; Pavelić, Krešimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 18th UICC International Cancer Congress in International Journal ofn Cancer / - Oslo : Wiley-Liss, 2002, 333-334

Skup
18th UICC International Cancer Congress

Mjesto i datum
Oslo, Norveška, 30.06.2002. - 05.07.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
NF1; colon cancer; loss of heterozygosity

Sažetak
Colorectal carcinomas are characterized by multiple genetic aberrations that occur during tumorigenesis. Several tumor suppressor genes associated with colorectal carcinoma have been identified: MCC and APC on chromosome 5q, p53 on chromosome 17p, nm23-H1 on chromosome 17q, and DCC and DPC4 on chromosome 18q. We examined 60 cases of human sporadic colon cancer and corresponding normal tissue samples to evaluate the loss of heterozygosity (LOH) at the NF1 gene loci. The purpose of this study was also to evaluate whether the LOH at the NF1 gene is associated with clinicopathological characteristics in sporadic colon cancer. DNAs were used for PCR, RFLP, VNTR, and LOH analysis. PCR was performed using specific pairs of primers. PCR products were analyzed by RFLP analysis, and VNTR analysis. To analyze LOH at the NF1 gene loci we used three polymorphic markers: one RFLP marker (exon 5 RsaI) and two VNTR markers (IVS27AAAT2.1 and IVS38GT53.0). Using these three polymorphic markers 50 (83.3%) patients were found heterozygous and informative for LOH analysis. DNA from 9 (18%) tumors exhibited LOH at the NF1 locus. The majority NF1 gene LOH was observed in Dukes' A (56%), in the well differentiated tumors (43%), and in the tumors that were ≤ 5cm (67%). Our results support the view that malignant progression is a consequence of more than one genetic change and suggest that inactivation of NF1 gene plays a role in a multistep process of colon tumor progression as an early event.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA