Naslov
ANALYSIS OF MICRONUCLEUS AND NUCLEAR ABNORMALITIES IN MUCOSA BUCCAL CELLS OF AGRICULTURAL WORKERS EXPOSED TO PESTICIDE MIXTURES IN TLAXCALA STATE, MEXICO

Autori
Sánchez-Alarcón ; Juana ; Pérez-Zempoalteca, Yuliana ; Milić, Mirta ; Montiel-González, JMR ; Valencia-Sánchez, RA ; Valencia-Quintana, Raphael

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
IUTOX 2016 – XIV International Congress of Toxicology (ICT) and X Mexican Congress of Toxicology / - , 2016

Skup
IUTOX 2016 – XIV International Congress of Toxicology (ICT) and X Mexican Congress of Toxicology

Mjesto i datum
Mérida, Meksiko, 02.10.2016. - 06.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pesticides; micronucleus buccal cytome assay; Mexico

Sažetak
Tlaxcala is a land of agriculture, which is why agricultural pest control is essential, and the most effective method is to use chemicals. However, they represent a significant source of occupational and non-occupational exposure to potentially toxic agents. Objetive: The aim of this study was to evaluate the geno- and cyto- toxic effects of pesticides in exfoliated buccal cells of workers occupationally exposed in Tlaxcala, Mexico. Materials and Methods: The micronucleus assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. The study compared 32 agricultural workers in the rural community of Tlaxcala, Mexico, with 30 non- exposed individuals. All of the individuals signed an informed consent form and filled in a detailed questionnaire enquiring into possible confounding factors (age, gender, smoking and drinking habits, vaccination, medication, X-ray examinations and diet). In the case of the affected group, years of exposure and protective measures used were also recorded. Buccal epithelium cell samples were collected during the day. The subjects rinsed their mouth with water before sampling, buccal cell samples were obtained by gently scraping the roof of the mouth. The slides were stained by the Feulgen reaction technique. 3000 epithelial cells of each individual were evaluated to determine MN, KR, KL, and BN frequencies. In order to compare differences among the exposed and non-exposed, Welch’s unequal variances t- test was applied. Results: The study revealed that the frequency of MN and the nuclear anomalies (KR, and BN), increased significantly in the exposed group. No significant effect on genetic damage was observed as a result of age, smoking, and alcohol consumption. Conclusions: This study provided valuable data for establishing the possible risk to human health associated with pesticide exposure.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita

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