Naslov
HCOMET cost action 15132 – an international network of researchers who are using comet assay in human biomonitoring studies

Autori
Milić, Mirta ; MC Members of hCOMET ; Steering Committee of ComNet

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Arhiv za higijenu rada i toksikologiju, Vol.67 No.Supplement / Kopjar, N ; Durgo, Ksenija - Zagreb, 2016, 29-29

Skup
Abstracts of the 5th Croatian Congress of Toxicology with International Participation CROTOX 2016

Mjesto i datum
Poreč, Hrvatska, 09.10.2016. - 12.10.2016

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
comet assay ; hCOMET ; DNA damage ; DNA repair ; networking

Sažetak
The single cell gel electrophoresis assay, also called Comet Assay is a method for measuring DNA strand breaks in single cells or nuclei. According to different setups and the use of specific enzymes and treatments, the assay can measure specific types of DNA damage and repair. Its in vivo alkaline version has been adopted as the OECD guideline TG 489 in 2014. Due to its potential as a predictive marker of disease and cancer risk, but also due to inter- laboratory variations in measuring DNA damage and repair, this network aims to create a unified database of comet assay data related to human health and disease. It will serve as a base for pooled analysis of DNA damage and repair in humans in order to give definitive information on the factors causing/preventing DNA damage, so as to establish standard protocols and guidelines that would help reduce inter-laboratory variations and through ring studies, training schools, workshops, publications, and creation of cohort of skilled molecular epidemiologists explore further the possibilities of use of this valuable technique in disease prevention and outcome. The network will continue the work of the previous network ComNet, and research and capacity building objectives of the network will be elaborated on through eight different Working Groups: creating a database, pooled analysis, ring studies, guidelines and SOPs, DNA repair, different cell types, planning for the future, and technical innovation and implementation. First results are expected at the beginning of 2017, and a new ICAW Workshop will be organised at the University of Navarra in August 2017.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA