Naslov
Assessment of DNA Damage by Comet Assay in Buccal Epithelial Cells: Problems, Achievement, Perspectives

Autori
Sánchez-Alarcón, Juana ; Milić, Mirta ; Gómez- Arroyo, Sandra ; Montiel-González ; J Mariano R ; Valencia-Quintana ; Raphael

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, pregledni

Knjiga
Environmental Health Risk - Hazardous Factors to Living Species

Urednik/ci
Larramendy, Marcelo L ; Soloneski, Sonia

Izdavač
IntechOpen

Grad
Rijeka

Godina
2016

Raspon stranica
77-135

ISBN
978-953-51-2402-3

Ključne riječi
: SCGE assay, buccal mucosa cells, genotoxic risk assays, DNA damage, comet assay

Sažetak
DNA damage risk assessment in comet assay by the use of buccal mucosa cells has great advantages incomparisonwithother celltype sampledue tomore safely, easier, cheaper, and non-invasive method for in vivo studies. According to the OECD Guidelines, the in vivo mammalian alkaline comet assay is well- established and validated method for measuring DNA strand breaks in single eukaryotic cells. Considering exposure to xenobiotics and endogenous damage inductors, buccal mucosa cells are the first to be in direct contact after exposure and this makes them an ideal biomatrices in evaluation of thelevelofindividualgenotoxicitytoseveralcompou ndsalreadymentioned.Theirclinical diagnostic applicability confers a potential use in patients across time. However, the number of publications referring to the human buccal comet assay is low in the last two decades. This low growing interest may be explained by several factors, including its relative technical problems. Different procedures have been used in collecting and processingthe samples.Inordertohavewidespreadacceptance andcredibilityinhuman populationstudies, thecometassayinbuccalcellsreq uiresstandardizationoftheprotocol, of parameters analyzed, and a better knowledge of critical features affecting the assay outcomes, including the definition of the values of spontaneous DNA damage. There is a need for further collaborative work as in the HUMN (micronucleus assay on lympho‐ cytes) and HUMNxL (micronucleus assay on buccal cells) collaborative projects. The creationofanetworkoflaboratorieswillallowmorefo cusedvalidationstudies, including the design of a classic, historic, prospective cohort study in order to explore the link between measures of genetic instability in the buccal mucosa and the risk of cancer and other chronic-degenerative diseases. One such network connection will start in 2016 as a COST project under the name “hCOMET—The comet assay as a human biomonitoring tool” launched by Prof. Andrew Collins.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita, Dentalna medicina

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