Naslov
Chemical composition and a-glucosidase inhibitory activity of plants traditionally used for management of diabetes in Croatia

Autori
Bljajić, Kristina ; Vujić, Lovorka ; Jablan, Jasna ; Zovko Končić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
BOOK OF ABSTRACTS • KNJIGA ABSTRAKTA The International Bioscience Conference and the 6th International PSU – UNS Bioscience Conference IBSC 2016 / Mimica-Dukić, Neda ; Pajević, Slobodanka ; Mandić, Anamarija - Novi Sad : University of Novi Sad, Faculty of Sciences, 2016, 209-209

ISBN
978-86-7031-364-4

Skup
The International Bioscience Conference and the 6th International PSU – UNS Bioscience Conference IBSC 2016

Mjesto i datum
Novi Sad, Srbija, 19.09.2016. - 21.09.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Diabetes; -glucosidase inhibitory activity; heavy metals; polyphenols.

Sažetak
INTRODUCTION: Type 2 diabetes affects 90% of diabetics and is largely the result of excess body weight and physical inactivity. The treatment consists of lowering blood glucose levels and preventing the development of secondary diabetic complications. OBJECTIVES: Treatment of type 2 diabetes by traditional medicine is common in Croatia. The aim of this study was to investigate potential usefulness of several plants traditionally used for management of diabetes by examining their chemical compositition and -glucosidase inhibitory activity. In order to eliminate the potential of influence of heavy metals on the enzyme activity, their amount in the samples was also determined. METHOD / DESIGN: For this study, 7 medicinal plants traditionally used in treatment of diabetes were selected: Artemisia absinthium (leaf), Achillea millefolium (aerial parts), Betula pendula (leaf), Centaurium erythraea (aerial parts), Morus sp. (leaves), Salvia officinalis (leaf) and Sambucus nigra (flower). Aqueous and ethanolic extracts of selected plants were prepared. Phenolic composition of the extracts was determined using RP-HPLC with UV and/or DAD detection, while -glucosidase inhibitory was determined spectrophotometrically Heavy metals concentration was determined using the bench top Total reflection X-ray Fluorescence (TXRF) spectrometer “S2 Picofox” (Bruker Nano GmbH, Berlin, Germany). RESULTS: HPLC analysis has shown that the selected medicinal plants contain a variety of phenolic acids while the investigated flavonoid derivatives were less common. Most of investigated samples (6 from 7) contained caffeic acid and/or its derivative, chlorogenic acid. Knowing that these cinnamic acid derivatives may, even in low concentrations, prevent glucose-induced endothelial dysfunction associated with inflammation and oxidative stress, it may be concluded that the plants that contain them may contribute to the prevention of secondary complications of diabetes. Furthermore, all the prepared extracts exhibited -glucosidase inhibitory activity. Among the investigated samples Morus sp and B. pendula extracts were the most active. In this investigation TXRF analysis was successfully applied for the multielemental analysis. The levels of investigated metals in all analyzed samples were in the permitted concentrations. CONCLUSIONS: Plants used for management of diabetes in Croatia possess -glucosidase inhibitory activity. Furthermore, they contain numerous bioactive polyphenols, caffeic acids and its derivatives being the most prominent examples. The content of heavy metals in all prepared extracts were bellow maximal permissible concentrations. The performed research indicates that the investigated medicinal plants may possess significant potential in treatment of diabetes and its complications.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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