Naslov
Biological activity of isolated compounds from Centaurea ragusina L.

Autori
Vujčić, Valerija ; Radić Brkanac, Sandra ; Radić Stojković, Marijana ; Žilić, Irena ; Tolić, Sonja ; Krivohlavek, Adela ; Ivanković, Siniša ; Ivanković, Dušica ; Stojković, Ranko ; Hrenović, Jasna ; Ruščić, Mirko ; Grienke, Ulrike ; Rollinger, Judith Maria

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Archives of Industrial Hygiene and Toxicology, Abstracts of CROTOX 2016 / Durgo, Ksenija - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2016, 42-42

Skup
The 5th Croatian Congress of Toxicology with International Participation, CROTOX 2016.

Mjesto i datum
Poreč, Hrvatska, 09.10.2016. - 12.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biological activity ; flavonoid ; sesquiterpene lactones interactions with polynucleotides

Sažetak
Antioxidant, antibacterial, and cytotoxic activity of three flavonoids (chrysin, oroxylin A, and hispidulin) and sesquiterpene lactones (hemistepsin A, deacylcynaropicrin and (3aR, 4S, 6aR, 8S, 9aR, 9bR)-[Dodecahydro-8- dihydroxy-3, 6, 9- tris(methylene)-2oxo-2(3H)- azuleno[4, 5-b]furanyl]-3-methyl-butanoate) isolated from ethanolic extract of the Croatian endemic plant species Centaurea ragusina L. were studied. The interactions of isolated compounds with double stranded polynucleotides ctDNA and poly A – poly U (possible biological targets) were studied with circular dichroism spectroscopy (CD spectroscopy) at pH=5.0 and pH=7.0 to provide a better understanding of the biological activity of the tested compounds. Flavonoid hispidulin showed moderate antioxidant activity measured by the ABTS method while other isolated compounds from ethanolic extract showed weak antioxidant activity. Two sesquiterpene lactones (3aR, 4S, 6aR, 8S, 9aR, 9bR)-[Dodecahydro-8-dihydroxy-3, 6, 9-tris(methylene)-2oxo-2(3H)-azuleno[4, 5- b]furanyl]-3- methyl-butanoate) and hemistepsin A showed significant antibacterial activity against Staphylococcus aureus ATCC25923 (MIC 31.3 g mL-1). All tested compounds showed weak antibacterial activity against Acinetobacter baumannii DURN. Among the isolated compounds, only (3aR, 4S, 6aR, 8S, 9aR, 9bR)-[Dodecahydro- 8-dihydroxy-3, 6, 9-tris(methylene)-2oxo- 2(3H)-azuleno[4, 5-b]furanyl]-3-methyl- butanoate) exerted selective and prominent tumour cell-growth inhibitory activity towards SCCVII cell line (IC50 = 2.55μM). Simultaneously, (3aR, 4S, 6aR, 8S, 9aR, 9bR)- [Dodecahydro-8-dihydroxy- 3, 6, 9- tris(methylene)-2oxo-2(3H)-azuleno[4, 5- b]furanyl]-3-methyl-butanoate) showed a weak interaction with DNA indicating that some other target/mechanism is responsible for its prominent cytotoxic activity towards SCCVII cell line.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

Napomena
Naziv projekta: Biološka aktivnost
fitokemikalija biljne vrste Centaurea ragusina
L.(BioFitoCen) Šifra projekta: HR.3.2.01-0290
Projekt sufinancira Europska unija: iz
Europskog socijalnog fonda u sklopu programa
Razvoj ljudskih potencijala 2007. – 2013.

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