Naslov
UVB-induced regulated necrosis is followed by autophagy and caspase activation only when FADD is deficient

Autori
Antunovic, Maja ; Caput Mihalic, Katarina ; Pusic, Maja ; Marijanovic, Inga

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
EMBO Conference on translational research in cancer cell metabolism

Mjesto i datum
Bilbao, Španjolska, 04.10.2016. - 06.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
regulated necrosis; autophagy; FADD; UVB

Sažetak
Research during the last decade clearly illustrates the important contribution of alternative forms of cell death in development, homeostasis and pathogenesis. Fas-associated protein with death domain (FADD) is critical adaptor protein for death receptor-mediated apoptosis. Recent evidence suggested negative roles of FADD in RIP1- and RIP3-dependent necroptosis. Since necroptosis can be initiated by various stimuli in a variety of cell types independently of death receptors, the exact mechanisms and functions of FADD require further investigation. Based on the presumption that FADD protein intermediates apoptotic, as well as necroptotic and autophagic signals after exposure to NB-UVB irradiation, we tested its role of death adaptor using FADD knockout mouse embryonic fibroblasts and specific inhibitors, Necrostatin- 1 and pan-caspase inhibitor zVAD-fmk. The results show that wild type mouse embryonic fibroblast die by triggering apoptotic death signals through mitochondrial control, but independently of p53. FADD knockout mouse embryonic fibroblasts die by regulated necrosis and autophagy, by activating caspase-3 and -9. Their necrotic program does not involve p53 nor Bax and Bcl-2. The results show that protein FADD as well as RIP1 are essential for triggering apoptotic cell death after NB-UVB irradiation. FADD protein acts as negative regulator of necroptosis followed by autophagy.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA