Naslov
Tff3 deficiency affects lipid metabolism

Autori
Bujak, Maro ; Tartaro Bujak, Ivana ; Mihalj, Martina ; Tolušić Levak, Maja ; Novak, Sanja ; Mihaljević, Branka ; Drenjančević, Ines ; Baus-Lončar, Mirela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International Conference on the Bioscience of Lipids / - , 2016, 62-62

Skup
International Conference on the Bioscience of Lipids

Mjesto i datum
Chamonix, Francuska, 04.09.2016. - 08.09.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Tff proteins ; fatty acid ; metabolism

Sažetak
Trefoil factor family proteins (Tff1, Tff2, Tff3) are small and compact peptides containing one or two so called trefoil domains. TFFs are predominanatly expressed in gastrointestinal tract where they have important role in maintenance of epithelial barrier integrity during the process of restitution upon mucosal injury. Moreover their role in other systems started to emerge. Tff3 gene was identified as one of the genes involved in liver steatosis. Hepatic Tff3 expression levels are decreased in obese and high-fat diet induce mice compared to control mice. The aim of this study was to determine hepatic neutral lipid accumulation, fatty acid profile in the liver and serum of Tff3 knock-out mice as well as the serum lipid content. Mice were anesthetized at the age of 12 weeks and blood was collected by cardiac puncture. Following mice were sacrificed by cervical dislocation and liver tissues were collected for histological analysis (HE, Oil red O). Total lipids for fatty acid profiling were extracted from tissue homogenates and serum which were analyzed by gas chromatography (Varian 450-GC). Serum lipids were analysed in routine laboratory using Architect c8000 (Abbott Diagnostic). Microscopic analysis of liver sections revealed normal tissue architecture (HE). Oil red O staining of liver sections from Tff3 deficient mice showed accumulated neutral lipids. Tff3 knock-out mice exhibited statistically changed level of numerous fatty acids in the liver and serum compared to WT. The level of saturated and polyunsaturated fatty acids were elevated and the level of monounsaturated fatty acids were decreased in liver of Tff3 deficient mice. In addition, the n-3/n-6 ratio was elevated in liver and decreased in serum of Tff3 deficient mice compared with wild type strain. However, total serum levels of the cholesterol, triglycerides, HDL or LDL cholesterol did not differ among the groups. Our results revealed that Tff3 is involved in fatty acid metabolism and as such it could be a potential target to develop therapeutic strategies against metabolic disorders.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA