Naslov
Natural deep eutectic solvents as beneficial extractants for enhancement of plant extracts bioactivity

Autori
Radošević, Kristina ; Ćurko, Natka ; Gaurina Srček, Višnja ; Cvjetko Bubalo, Marina ; Tomašević, Marina ; Kovačević Ganić, Karin ; Radojčić Redovniković, Ivana

Izvornik
LWT-Food Science and Technology (0023-6438) 73 (2016); 45-51

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Antiproliferative activity ; Natural deep eutectic solvents ; Grape skin ; Green extraction ; Phenolics

Sažetak
In the present study phenolic grape skin extracts were prepared by using five choline chloride based natural deep eutectic solvents (NADESs) containing glucose, fructose, xylose, glycerol, malic acid and valorised by testing their biological activity in vitro using two human tumour cell lines (HeLa and MCF-7). Initially, used NADESs were investigated regard to their toxicity and low cytotoxicity of solvents was observed toward HeLa and MCF-7 cells (EC50 values > 2000 mg/L). Among used choline chloride based NADESs, the one containing malic acid showed the best performance concerning extraction efficiency (total phenolic and total anthocyanin were 91 and 24 mg/g dw), as well as antioxidant (ORAC values were 371 μmol TE/g dw) and antiproliferative activity (percentage of cell viability were about 20%). Herein, for the first time it was showed that NADES components could be chosen not only to fine-tune solvent physicochemical characteristics but also to enhance biological activity of extracts prepared in NADESs. Therefore, our research confirmed that NADESs are excellent and promising choice of solvents for sustainable and green extraction, which will lead to its novel application in food and pharmaceutical industry.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

POVEZANOST RADA