Naslov
Impact of L-arginine (a nitric oxide synthesis precursor), and N-omega-nitro-L-arginine- methyl- ester (L-NAME) (a non-selective nitric oxide synthase inhibitor) Chronic, Subchronic, and Acute Toxicity-Induced Lesions on Ascites– Pulmonary Hypertension Syndrome Development in Broiler Chickens
(Impact of L-arginine (a nitric oxide synthesis precursor), and N-omega-nitro-L-arginine- methyl-ester (L-NAME) (a non-selective nitric oxide synthase inhibitor) Chronic, Subchronic, and Acute Toxicity-Induced Lesions on Ascites– Pulmonary Hypertension Syndrome Development in Broiler Chickens)

Autori
Tišljar, Marina ; Grabarević, Željko ; Artuković, Branka ; Džaja, Petar ; Foršek, Jadranka ; Barić Rafaj, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of 3rd International Veterinary Congress, August 18-20, 2016, conferenceseries.com 625th Conference, London, UK: Conference Series LLC, USA ; Conference Series, UK , 2016. 84-84. u:Journal of Veterinary Science and Technology, 7 (5), 84 (Suppl), August 2016, Open Access / Fujisaki, K. ; Memon, M. A. ; Gurbuz, Y. - London : Delhi : OMICS International, 2016, 84-84

Skup
3rd International Veterinary Congress August 18-20, 2016 London, UK

Mjesto i datum
London, Ujedinjeno Kraljevstvo, 18.08.2016. - 20.08.2016

Vrsta sudjelovanja
Ostalo

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
nitric oxide; broilers; ascites - pulmonary hypertension syndrome; L-arginine; L-NAME

Sažetak
Experiment A. Chronic toxicity. 140 one day-old male broiler chicks divided in four groups. Every other day: intraperitoneal (ip) L-NAME (LN, 10 mg/kg BW) ; L-arginine (LA, 100 mg/kg BW), L-arginine and a L-NAME combination (100 and 10 mg/kg BW, respectively), and physiological saline (0.90% w/v ; 0.5 mL/kg BW). Seven birds were euthanized weekly during their first five weeks of life. Experiment B. Subchronic toxicity. Equal doses of the same substances: ip every eight hours into 38 day- old birds over 36 hours. Experiment C. Acute toxicity. Thirty day-old birds: one ip dose of various concentrations of L-NAME (50, 100 and 150 mg/kg BW) ; L-arginine (100 mg/kg BW) and saline (0.5 ml/kg BW). All the birds were euthanized after six hours. The end of experiment A: the gross finding of ascites- pulmonary hypertension syndrome (PHS) confirmed vasoconstrictory effect of L-NAME in five birds (LN). Toward the end of experiment A ; in experiment C: histopathological findings (myocardial/pulmonary oedema /LN/ ; congestion/ haemorrhages /LA/) the most prominent (higher LN-doses /100 and 150 mg/kg BW/). Irreversible myocardiolysis and hepatocellulolysis: confirmed in all three experiments (LN). Focal myocardial degeneration solely: in the L- NAME/L-arginine simultaneously treated group. Haematological and blood chemistry values ; stress index value ; and relative organ weights agreed with well-known literature data on PHS. Experiments A and C. The severity of LN- provoked hypoxic changes and plentiful haemorrhages (LA) - dose and time dependent. Mild changes in L-NAME/L-arginine group confirmed a protective role of LA.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Veterinarska medicina

POVEZANOST RADA