Naslov
Macroporous PLA construct supporting the osteoinductive porous chitosan-hydroxyapatite hydrogel for bone tissue engineering

Autori
Rogina, Anamarija ; Pribolšan, Lidija ; Gomez-Estrada, Luis ; Gallego Ferrer, Gloria ; Marijanović, Inga ; Ivanković, Marica ; Ivanković, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The International Journal of Artificial Organs / Remuzzi, Andrea - Milano : Wichtig, 2016, 326-326

Skup
XLIII Congress of the European Society for Artificial Organs

Mjesto i datum
Varšava, Poljska, 14.09.2016. - 17.09.2016

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Poly(lactic acid); Chitosan; Hydroxyapatite; Compressive strength; HMSCs differentiation
(poly(lactic acid); chitosan; hydroxyapatite; compressive strength; hMSCs differentiation)

Sažetak
Chitosan-hydroxyapatite based composites have proved to exhibit great biocompatibility, minimal foreign body reaction and good wettability, although biomechanical properties can not meet applications in vivo1, 2. To overcome the mechanical drawback, additional synthetic polymers such as poly(lactic-acid) (PLA) is used. The aim of this research is to produce a three-component system with suitable microstructure, biocompatibility, bioactivity and mechanical properties for bone tissue defect repair. Material’s preparation is divided in three steps: 1) 3D printing of PLA scaffold ; 2) in situ synthesis of chitosan-hydroxyapatite (CHT-HA) suspension ; 3) vacuum impregnation of PLA scaffold with CHT-HA suspension, and finally freeze-gelation of PLA/CHT-HA scaffold. The scaffold’s composition and microstructure were investigated by FTIR spectroscopy, X-ray and DSC analysis and SEM imaging. Osteogenic properties were evaluated by in vitro cell culture of hMSCs during 21 days using quantitative polymerase chain reaction. The X-ray and FTIR identification has confirmed successful in situ formation of hydroxyapatite within chitosan matrix of PLA/CHT-HA scaffold. Freeze-gelation has provided highly porous structure of CHT-HA within PLA scaffold with good pore interconnectivity, confirmed by SEM imaging. The proliferation of hMSCs cultured on PLA/CHT-HA scaffold during 7 and 14 days indicates favourable microenvironment. Quantitative analysis of gene expression measured at 21 day of culture confirmed better osteogenic properties of PLA/CHT-HA scaffold indicating hydroxyapatite influence in osteogenesis stimulation. The combination of 3D printing and freeze-gelation has provided production of highly porous scaffold suitable for cell proliferation. In situ precipitation of calcium phosphate as bioactive component is suitable for preparation of more bioresorbable hydroxyapatite. Positive osteogenic signal of PLA/CHT-HA scaffold highlights the potential use in bone reconstruction

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo, Temeljne tehničke znanosti

POVEZANOST RADA