Naslov
Experimental modification of 4-hydroxynonenal-protein conjugates in rat brain in vitro by neuroprotective pyri-doindole derivative stobadine

Autori
Lončarić, Iva ; Stolc, Svorad ; Žarković, Kamelija ; Vuković, Tea ; Matijević, Tanja ; Waeg, Georg ; Žarković, Neven

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
First International Meeting of the HNE-Club: 4-hydroxynonenal and other lipid peroxidation products : Book of abstracts / - Salzburg, 2002, 52-52

Skup
International Meeting of the HNE-Club: 4-hydroxynonenal and other lipid peroxidation products (1 ; 2002)

Mjesto i datum
Salzburg, Austrija, 13.07.2002. - 15.07.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
4-hydroxynonenal; stobadine

Sažetak
Stobadine (STB), novel pyridoindole derivative which scavenges hydroxyl, superoxide, alkoxyl and peroxyl radicals, and quench singlet oxygen has been proved neuroprotective in many experimental models. Since, in clinic preventive administration is not possible, the aim of our study was to determine if STB acts as neuroprotectant after onset of ischaemic brain damage and consequential generation of lipid peroxidation product 4-hydroxynonenal (HNE). Immunocytochemical analysis of HNE-protein conjugates, using monoclonal antibodies specific for the HNE-histidine epitope, was performed on isolated rat brain cells after ischaemic brain damage induced by occlusion of the common carotid arteries and additional in vitro incubated with STB and/or HNE. STB decreased formation of HNE-protein conjugates in brain cells which were isolated from "normo-oxigenated" brain area but not in the brain cells isolated from ischaemic brain. When stobadine was added to cell cultures of human neuroblastoma IMR32, prior or after HNE, cell-ELISA method showed decrease in HNE positivity. This indicates that STB can be effective early after onset of oxidative stress, when damage of brain cells might be reversible.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA