Naslov
Changes in the IgG glycome associated with inflammatory bowel disease

Autori
Keser, Toma ; Trbojević Akmačić, Irena ; Ventham, Nicholas T ; Theodoratou, Evropi ; Vučković, Frano ; Kennedy, Nicholas A ; Nimmo, Elaine R ; Kalla, Rahul ; Drummond, Hazel ; Štambuk, Jerko ; Campbell, Harry ; Hedin, Charlotte ; D'Amato, Mauro ; Halfvarsson, Jonas ; Satsangi, Jack ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Glycobiology / Haltiwanger, Robert S. - : Oxford University Press, 2015, 1242-1242

Skup
2015 Annual Meeting of The Society for Glycobiology

Mjesto i datum
San Francisco (CA), Sjedinjene Američke Države, 01.12.2015. - 04.12.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Inflammatory bowel disease; Glycomics; IgG glycans; Ulcerative colitis; Crohn’s disease

Sažetak
Glycobiology is an underexplored research area in inflammatory bowel disease (IBD), and glycans are relevant to many aetiological mechanisms described in IBD. Alterations in N- glycans attached to the IgG Fc fragment can affect molecular structure and immunological function. Recent genome-wide association studies reveal pleiotropy between IBD and IgG glycosylation. We conducted two studies to better understand changes in the IgG glycome associated with IBD. In the first study we analyzed IgG glycans in 507 patients with ulcerative colitis (UC), 287 patients with Crohn’s disease (CD) and 320 controls. Statistically significant differences in IgG glycome composition between patients with UC, or CD, compared to controls, were observed. Both UC and CD were associated with significantly decreased IgG galactosylation (digalactosylation, UC Odds ratio [OR]=0.71, 95% confidence interval[CI] 0.5-0.9, p=0.01, CD OR=0.41, CI 0.3-0.6, p=1.4x10-9) and significant decrease in the proportion of sialylated structures in CD (OR=0.46, CI 0.3- 0.6, p=8.4x10-8). Logistic regression models incorporating measured IgG glycan traits were able to distinguish UC and CD from controls (UC p=2.13x10-6, CD p=2.20x10-16), with receiver operator characteristic curves demonstrating better performance of the CD model (Area under curve[AUC]=0.77) over the UC model (AUC=0.72) (p=0.026). The observed differences indicate significantly increased inflammatory potential of IgG in IBD. In the second study we analyzed IgG glycans in 87 twin pairs discordant for IBD, 16 twin pairs with IBD and 103 healthy controls. The results indicate that healthy twins are more similar to healthy controls than to their diseased twins.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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