Naslov
Mannosylated liposomes with built-in peptidoglycan based immunomodulators for subunit vaccines formulations

Autori
Štimac, Adela ; Frkanec, Ruža ; Frkanec, Leo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstract of the Congress of the Croatian Society of Biochemistry and Molecular Biology on the Occasion of the 40th Anniversary / Katalinić, Maja ; Kovarik, Zrinka - Zagreb, 2016, 105-105

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology on the Occasion of the 40th Anniversary

Mjesto i datum
Split, Hrvatska, 01.06.2016. - 04.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
liposomes ; targeted delivery ; immunomodulator ; mannose receptor

Sažetak
This study is focused on the preparation of mannosylated liposomes with built-in small molecule immunopotentiators for targeted, receptors mediated, delivery of antigens and adjuvants. The liposomes are recognized as attractive drug delivery carriers characterized by the absence of toxicity and low intrinsic immunogenicity. Mannose receptors (MR) have very important role in a large variety of cellular recognition processes, particularly those with immune function. MR present on the cell surface of different immunocompetent cells such as macrophages and dendritic cells, are considered to be pattern-recognition receptors. Therefore, they could be responsible for the receptor mediated uptake of mannosylated antigens, biologically active molecules containing mannose or mannose targeted drug delivery systems.1, 2 In the continuation of our studies, our interest is in finding a potent targeted delivery vehicle for drugs, especially for subunit vaccines.3 Mannosylated liposome preparations with incorporated model antigen, ovalbumin (OVA), and immunomodulators, PGM and Ad2TP2, were prepared and characterized. The influence of mannosylated liposomal formulations of antigen and immunomodulators on the specific immune reaction in mice was tested. The liposomes were mannosylated in two ways, by covalent attachment of p-aminophenyl-α-D-mannopyranoside to the preformed liposomes and by incorporation of synthetic di- and tetramannosyl- lipoconjugates into the lipid bilayer of liposomes. The coupling efficiency of p-aminophenyl-α-D-mannopyranoside to the preformed liposomes and entrapment efficiency of OVA and immunomodulators into mannosylated liposomes were measured spectrophotometrically and by using HPLC. Evaluation of adjuvant activity of mannosylated liposomes with built-in immunomodulators in vivo, has shown their significant immunomodulatory effect. 1. C. Kelly, C. Jefferies, S.-A. Cryan, Journal of Drug Delivery 2011 (2011), Article ID 727241. 2. J. M. Irache, H. H. Salman, C. Gamazo, S. Espuelas, Expert. Opin. Drug. Deliv. 5 (2008) 703-724. 3. A. Štimac, S. Šegota, M. Dutour Sikirić, R. Ribić, L. Frkanec, V. Svetličić, S. Tomić, B. Vranešić, R. Frkanec, Biochim. Biophys. Acta 1818 (2012) 2252-2259

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

POVEZANOST RADA