Pregled bibliografske jedinice broj: 829281
Tripeptide hydrogelator (Ac-FFA-NH2) as a model of binding site Aβ-protein ; comparative binding studies with Thioflavin-T and other Aβ- binders
Tripeptide hydrogelator (Ac-FFA-NH2) as a model of binding site Aβ-protein ; comparative binding studies with Thioflavin-T and other Aβ- binders // SysChem 2016, Abstract Book / Radmila Řápková, Pavel Drašar (ur.).
Prag: Chemicke listy, Association of Czech Chemical Societies,, 2016. str. 126-126a (predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 829281 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Tripeptide hydrogelator (Ac-FFA-NH2) as a model of binding site Aβ-protein ; comparative binding studies with Thioflavin-T and other Aβ- binders
Autori
Pospišil, Tihomir ; Žinić, Mladen ; Frkanec, Leo
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
SysChem 2016, Abstract Book
/ Radmila Řápková, Pavel Drašar - Prag : Chemicke listy, Association of Czech Chemical Societies,, 2016, 126-126a
Skup
SysChem 2016, CMST COST Action CM1304 Emergence and Evolution of Complex Chemical Systems
Mjesto i datum
Valtice, Čehoslovačka, 08.05.2016. - 12.05.2016
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Aβ-amyloid aggregates; Supramolecular self assembly; Low molecular weight peptidic gelators; Binding of histological dyes
Sažetak
Series of tripeptide FFA derivatives was synthesized and tested for gelation of water and organic solvents. Only Ac-FFA-NH2 tripeptide exhibited gelation of water by self- assembly under physiological conditions. TEM of the Ac- FFA-NH2 hydrogel and the methanol/water gel showed the presence of straight fibers with relatively uniform diameters of around 50 nm. FTIR and NMR investigation pointed toward the cross-β structure type of hydrogen bonding of the tripeptide in the gel aggregates. Conjugated dyes (Thioflavin T and Congo Red) are commonly used to stain the plaques in histopathological studies3. Fluorescence titration of Ac-FFA-NH2 aqueous solution bellow its minimal gelation concentration with Thioflavin T (ThT) showed increase of ThT emission with increased tripeptide concentration and formation of the 1 : 1 complex with significant association constant.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
POVEZANOST RADA
Projekti:
098-0982904-2912 - Samo-udruživanje u gelovima i sinteza funkcionalnih hibridnih materijala (Frkanec, Leo, MZOS ) ( CroRIS)
HRZZ-IP-2013-11-7387 - Supramolekulska sinteza samo-organizirajućih funkcionalnih nanomaterijala i kompleksnih kemijskih sustava (SInFONIA) (Frkanec, Leo, HRZZ - 2013-11) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb
