ࡱ> :<9[ bjbj >*ΐΐ TG YpSSSSS.D P$ k#X..XXSSPPPX,SSPXPPPSPsIeLP)0YP!$!$PP!$`dPXXXPXXXYXXXX!$XXXXXXXXX : THE OLM (Proteus anguinus) IN CROATIA WHAT WE HAVE LEARNED TILL NOW Maja Lukac, DVM, PhD,1* Ivan Cizelj, dipl ing agr,2 Susanne Holtze, DVM, PhD,3 Dusan Jelic, mag edu biol, PhD,4 Frank Mutschmann, DVM, Dipl ECZM,5 Danijela Horvatek Tomic, DVM, PhD,1 Estella Prukner-Radovcic, DVM, PhD, Dipl ECPVS,1 Zeljko Gottstein, DVM, PhD,1 Ivan Rychlik, RNDr, PhD,6 Petra Videnska, Mgr, PhD,6 and Thomas B Hildebrandt, DVM, PhD, Dipl ECZM3 1Faculty of Veterinary Medicine, University of Zagreb, Zagreb 10000, Croatia; 2Zagreb Zoo, Zagreb 10000, Croatia; 3Leibniz Institute for Zoo and Wildlife Research, Berlin 10315, Germany; 4Croatian Institute for Biodiversity, Croatian Herpetological Society, Zagreb 10000, Croatia; 5Exomed, Berlin 10319, Germany; 6Veterinary Research Institute, Brno 62100, Czech Republic Proteus anguinus in Croatia conservation research project is aimed to collect information about the olms, to help protect this very unique and endangered aquatic amphibian living in underground carst. The field research of population density, using novel eccological research methods revealed the largest single population in the Markarova cave, with approx. one individual/m2, the highest density reported so far. Keeping the animals in captivity, in a specially designed cold room which meets all their needs regarding the temperature, water conductivity, aeration and food, showed that they accepted all the offered food, primarily the Lumbricus sp., with the preference of live food over frozen one. Microbiological results of skin, cloacal and oral cavity swabs revealed mostly the presence of soil and water microorganisms such as Aeromonas hydrophila and Aspergillus flavus, alredy described as pathogens in immuncompromised amphibians. Maldi-ToF method allowed identification of the Janchtinobacterium lividum, a bacterium with known anti-fungal properties. Next generation sequencing revealed the incorporation of Methylotenera sp. and Methyloversatilis sp. in the skin and oral cavity. Feces and intestinal scrapings were free of parasites. Real-Time PCR gave negative results for Batrachochytrium dendrobatidis, Batrachochytrium salamandrivorans and Ranavirus in skin swabs and for Clamydia sp. in oral cavity and cloacal swabs. Scanning of animals with a precise ultrasound machine was helpful in sex determination, general health assessment and early diagnosis and treatment of otherwise fatal Saprolegnia infections in captive animals. 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G 2!xx9PROTEUS ANGUINUS IN CROATIA  WHAT WE HAVE LEARN TILL NOWAdminKorisnikOh+'0  , L X dpx<PROTEUS ANGUINUS IN CROATIA WHAT WE HAVE LEARN TILL NOWAdminNormal Korisnik9Microsoft Office Word@d@b!e@8e՜.+,0$ hp  Home  :PROTEUS ANGUINUS IN CROATIA WHAT WE HAVE LEARN TILL NOW Title  !"#$%&'(*+,-./02345678;Root Entry F@Ie=1Table!$WordDocument>*SummaryInformation()DocumentSummaryInformation81CompObjy  F'Microsoft Office Word 97-2003 Document MSWordDocWord.Document.89q