Naslov
Pulsed Laser Ablation of Au in Media for in situ Nanoparticle Uptake into Cancer Cells

Autori
Nikša Krstulović, Fergal O’Reilly, Rasoul Al- Majmaie and Dominic Zerulla

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
COST MEETING TD1208

Mjesto i datum
Bertinoro, Italija, 13.09.2015. - 17.09.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Laser synthesized nanoparticles; cancer cells; nanoparticles uptake; in situ treatment

Sažetak
Over the last decade pulsed laser ablation in liquids been demonstrated as a promising non- chemical alternative technique for nanoparticle synthesis. Moreover, laser ablation in liquids allows fabrication of single-element or composite nanoparticles (with wide application in materials science, optical sensing, photovoltaic industry, complex plasmonic systems) and bio-functionalized (bio- conjugated) nanoparticles with wide variety of applications in nano-bio technology. Particularly in bio-applications the assessment and qualification of nanoparticles as agents for drug delivery is limited at least in part by the complexities of producing pure nanoparticle samples uncontaminated by the chemical production process residues. In comparison to standard chemical techniques, laser ablation in liquids provides chemically pure synthesis (green synthesis) of nanoparticles formed only of ablated material and not affected by the process impurities. In our proof-of-concept work gold nanoparticles synthesized by laser ablation in media were used to be taken up in situ by the cancer cells (SW480). Namely, the cells were placed in the same well plate and in the same time while ablation takes place. Nascent Au nanoparticles were then taken up by the cells during and after the ablation with relatively high efficiency and viability compared to the control case where no ablation was performed. We showed that freshly synthesized pure Au nanoparticles (contamination-free - no stabilizing agents) can be effectively delivered to the cells during and immediately after the synthesis by laser ablation preventing agglomerations and possible cell death by the interaction with agglomerates. This approach opens a possibility to synthesize nanoparticles from wide variety of materials to be taken up by cells in real-time (during synthesis) and possibly in-vitro. Further functionalization of the nanoparticles synthesized by laser ablation in media is possible by adding different molecules to the media thus making this approach versatile in terms of materials and properties of the nanoparticles pushing cells treatment (drug delivery, diagnosis and therapy) towards new frontiers of personalized medicine.

Izvorni jezik
Engleski

Znanstvena područja
Fizika

POVEZANOST RADA