Naslov
Reg genes expression in regenerating skeletal muscle

Autori
Starčević-Klasan, Gordana ; Ivanac, Danijel ; Jurišić-Eržen, Dubravka ; Jerković, Romana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts - The 6th International Symposium of Clinical and Applied Anatomy / Zoričić-Cvek, Sanja - , 2014, 25-26

Skup
The 6th International Symposium of Clinical and Applied Anatomy

Mjesto i datum
Malinska, Hrvatska, 26.06.2014. - 29.06.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Reg gene; Skeletal muscle; Regeneration
(Reg gene; skeletal muscle; regeneration)

Sažetak
The Reg genes have been well documented to play an important role in various tissue regeneration processes, cell proliferation, differentiation and carcinogenesis. However, the regulation of Reg gene expression in rat skeletal muscles regeneration process is poorly understood. Therefore, the aim of this investigation was to reveal the transcriptional activation of Reg genes in the skeletal muscles in response to injury. In 2-month-old Wistar rats three experimental models of muscle and nerve injury were used. The regeneration process was induced in both types of muscles, slow (m. soleus, SOL) and fast (m. extensor digitorum longus muscle, EDL) by injection of 1 ml of local anesthetic, by denervation while in third experimental model muscle denervation and injection of local anesthetic were combined. The RT-PCR analysis was performed for detection of Reg1, Reg3G and Reg4 mRNA in regenerating muscles. RT-PCR analysis showed that Reg1 gene is expressed in EDL and SOL with peak expression 12 hours after injury and signal intensity was constant till 7th day in all three experimental models. Reg3G can be detected over a longer period in comparison with Reg1 and signal intensity was lower. There was no detectable Reg4 mRNA expression in either SOL or EDL muscle. Our investigation revealed presence of Reg1 and Reg3G genes in regenerating skeletal muscle and its expression is up regulated with regeneration process. Reg1 and Reg3G genes expression was induced in both skeletal muscles at a considerably early time point after muscle injury. Our results imply that Reg genes have a significant role at the early stage of skeletal muscle regeneration.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA