Naslov
Spectrophotometric determination of Malondialdehyde in urine suitable for epidemiological studies

Autori
Weitner, Tin ; Inić, Suzana ; Jablan, Jasna ; Gabričević, Mario ; Domijan, Ana-Marija

Izvornik
Croatica chemica acta (0011-1643) 89 (2016), 1; 133-139

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
malondialdehyde ; thiobarbituric acid assay ; urine ; interferences ; stability.

Sažetak
A reliable method for spectrophotometric determination of urinary malondialdehyde (MDA), according to the thiobarbituric acid (TBA) assay, is described. To account for matrix interference and differences in individual urine composition, standard addition procedure was applied. The method is adequately selective (LoQ = 0.09 μM in the presence of 0.1 M creatinine and 0.5 M urea) and reliable (within- day and between-day variability of less than 5 %). The mean level of urinary MDA was 1.52 ± 0.73 μM that is in good agreement with spectrofluorometric determination (1.20 ± 0.56 μM ; p = 0.085) as well as with previous studies that used HPLC. Furthermore, it is demonstrated that MDA is stabile in urine at room temperature for 24 h and when stored at –20 °C for 6 months. The described method enables simple, rapid and cost-effective determination of urinary MDA as a relevant and non-invasive marker of “whole-body” oxidative stress.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA