Naslov
Multilevel ecotoxicity assessment of environmentally relevant bisphenol A concentrations using the soil invertebrate Eisenia fetida

Autori
Babić, Sanja ; Barišić, Josip ; Bielen, Ana ; Bošnjak, Ivana ; Sauerborn Klobučar, Roberta ; Ujević, Ivana ; Strunjak-Perović, Ivančica ; Topić Popović, Natalija ; Čož-Rakovac, Rozelindra

Izvornik
Journal of hazardous materials (0304-3894) 318 (2016); 477-486

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Bisphenol A ; Eisenia fetida ; filter paper contact test ; oxidative stress ; histopathology

Sažetak
Bisphenol A (BPA) presents a serious threat to soil ecosystems, yet its effects on soil- inhabiting organisms are mostly unexplored. Therefore, the impact of environmentally relevant BPA concentrations on a terrestrial model organism, the earthworm Eisenia fetida, was assessed. Animals were cutaneously exposed to 100 nM and 10 μM BPA up to 10 days (10-d). Next, a battery of biomarkers was used for ecotoxicological evaluation on a cellular, tissue and behavioural level. HPLC analysis showed that after a 10-d exposure, BPA accumulation reached a maximum of 2.50 μg BPA per g of wet tissue weight. On the cellular level, up to 3-d BPA exposure caused increased lipid oxidation indicating oxidative stress. Histopathological assessment of cell wall and ovaries after 7- and 10-d BPA exposure showed multiple abnormalities, i.e. hyperplasia of epidermis, increased body wall thickness and ovarian atrophy. Detection of these changes was facilitated by a newly proposed semi- quantitative scoring system. Finally, behavioural changes were detected after only 3 days of exposure to 100 nM BPA. Altogether, the presented multilevel toxicity evaluation indicates high sensitivity of earthworms to low BPA doses.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

Napomena
Authors equally contributed.

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