Naslov
NON-THERMAL EFFECT OF 1800 MHz RADIATION ON HUMAN NEUROBLASTOMA CELL

Autori
Marjanovic, Ana Marija ; Pavicic, Ivan ; Trosic, Ivancica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology / Katalinić, Maja ; Kovarik, Zrinka - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2016, 90-90

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology

Mjesto i datum
Split, Hrvatska, 01.04.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RF radiation; SH-SY5Y cells; GTEM cell; ROS

Sažetak
Aim of this study was to assess possible connection between short-term non-thermal RF exposure and development of oxidative stress. Human neuroblastoma cells (SH-SY5Y) were exposed to GSM modulated 1800 MHz radiation for 10, 30 and 60 minutes inside of Gigahertz Transversal Electromagnetic Mode Cell (GTEM). Electric field strength inside GTEM cell was 30 V/m and specific absorption rate (SAR) was calculated to be 1.6 W/kg. Cellular viability was measured by 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide assay (MTT) and level of reactive oxygen species (ROS) was determined by fluorescent probe 2′, 7′-dichlorofluorescin diacetate (DCFH-DA). Lipid oxidative damage in the cell samples was assessed by measuring concentrations of malondialdehyde (MDA). Viability of exposed cells remained within normal physiological values regardless of exposure duration. Compared to control, level of ROS in irradiated cell samples was found to be significantly higher for every exposure time. Increased concentration of MDA (p<0.05) was observed after 60 minutes radiation exposure. In conclusion, results of our study suggest that short-term exposure to 1800 MHz RF radiation increases ROS production in neuroblastoma cells causing oxidative damage to cell membranes

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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