Naslov
Electrochemical imaging of cell adhesion

Autori
Svetličić, Vesna ; Hozić, Amela ; Žutić, Vera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
46th Annual Meeting of the Biophysical Society ; u: Biophysical Journal 82 (2002) (S) / Moore, Peter B. - Bethesda (MD) : Biophysical Society, 2002, 630a-630a

Skup
Annual Meeting of the Biophysical Society (46 ; 2002)

Mjesto i datum
San Francisco (CA), Sjedinjene Američke Države, 23.02.2002. - 27.02.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cell adhesion; electrochemical imaging

Sažetak
We present the amperometric response of single cell adhesion in a real time, from the initial attachment to a finite state of spread cell. The technique is based on measurement of double-layer charge displacement at the mercury drop electrode. The flow of compensating current reflects the dynamics of adhesive contact formation and subsequent spreading of a cell. The spike-shaped signals have the peak current in microampere range, duration in milliseconds, and displaced charge in the nanocoulomb range. The only hypothesis used in interpreting the signals is the validity of the electrical double-layer model. A surprising similarity to adhesion signals of droplets of liquid hydrocarbons (C12-C18) suggests that collective properties of cell exterior govern the dynamics of adhesion and rate of spreading, with the fluidity playing a major role. The characteristic potential range of adhesion can serve to study the interplay of complex surface forces involved in cell-electrode double-layer interactions.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA