Naslov
MCMV pathogenesis in neonatal mice

Autori
Trgovcich, Joanne ; Crnković, Irena ; Krmpotić, Astrid ; Zorica, Irena ; Jonjić, Stipan ; Koszinowski, Ulrich

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th International Cytomegalovirus Workshop : Program and Abstracts / Pass, Robert F. - Orange Beach (AL) : UAB School of Medicine, 1997, A16-A16

Skup
6th International Cytomegalovirus Workshop

Mjesto i datum
Orange Beach (AL), Sjedinjene Američke Države, 05.03.1997. - 09.03.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
MCMV

Sažetak
Congenital and perinatal infections of CMV are a significant cause of morbidity and mortality in infants. The pathogenesis of these infections are not clearly understood. We have examined the disease progression of immunocompetent Balb/c newborn mice infected with MCMV. Mice were inoculated with 1000 PFU of wild-type MCMV, sacrificed on days 7, 14 and 21 days post-infection (p.i.) and tissues were harvested for titration and histopathological analysis. At this dose, 67% mortality was observed within the first 2 weeks of life. High virus titers were detected in numerous organs and tissues at 7 days p. i., which reflected an acute disseminated disease course notably involving fibroblast, muscle, epithelial and neuronal tissues. In most cases virus titers fell below detectable limits by days 14 and 21. Interestingly, the extent of pathogenetic changes increased over time and were maximal in most organs and tissues at 21 days p. i.. Late lesions were associated with inflammatory cell infiltrates suggesting that immunopathology may be an important feature of infection. To investigate viral determinants which influence virulence in this model, newborn mice were injected with virus strain MC95.21 (harboring a deletion of the m152 gene) or MC95.16 (harboring a deletion of the fcr 1 gene). These strains were strikingly attenuated in newborns compared to MCMV, inducing only 5-7% mortality with deaths occurring in the 3rd week of life. These deletion strains were variably restricted for growth in vivo compared to wild-type virus, and this correlated with less severe histopathological changes. Similar to wild-type infection, inflammatory lesions predominated at late times. Immunohistological and immunodepletion studies are underway to explore tha nature of the pathogenetic changes observed.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA