Naslov
Influence of Dipeptidyl-peptidase IV Deficiency on the Process of Cutaneous Wound Healing in a Streptozotocine-induced Model of Diabetes in Mice

Autori
Batičić Pučar, Lara ; Pernjak Pugel, Ester ; Detel, Dijana ; Varljen, Jadranka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of The Congress of the Croatian Society of Biochemistry and Molecular Biology / Katalinić, Maja ; Kovarik, Zrinka - Zagreb, 2016, 68-68

Skup
HDBMB2016

Mjesto i datum
Split, Hrvatska, 01.06.2016. - 04.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dipeptidyl-peptidase IV (DPP IV/CD26) ; Wound Healing ; Diabetes ; Streptozotocine-induced Model

Sažetak
Introduction: Dipeptidyl-peptidase IV, or CD26 molecule (DPP IV/CD26) is a multifunctional ubiquitous protein involved in various biological processes, including regulation of glucose homeostasis. Its role in mechanisms involved in the process of wound healing was indicated but pathophysiological processes of wound healing in hyperglycemia are insufficiently understood. We hypothesized that DPP IV/CD26 plays an important role in the process of wound healing in experimental hyperglycaemia conditions. Aim: The aim of this study was to determine whether the deficiency of DPP IV/CD26 influences processes that occur during wound healing in conditions of experimental hyperglycaemia. Materials and methods: Experimental diabetes was induced in CD26-/- and C57BL/6 mice by intraperitoneal administration of streptozotocine solution (50mg /kg for 5 days). Experimental wounds were induced mechanically on the dorsal part of diabetic and healthy animals. Mice were sacrificed the second, fourth, seventh and tenth day of healing of injured tissue. Histomorphometric, histopathological and immunohistochemical analyses of wound and control skin tissue samples were done. Serum DPP IV/CD26 in wild- type animals during the process of wound healing was measured spectrophotometrically. Results: During the process of wound healing in diabetes, corium regeneration started earlier in CD26-/- mice and the proliferation of epidermal cells was more pronounced. Furthermore, in CD26-/- animals the inflammatory phase lasted shorter and the number of T cells in the corium was significantly lower (p < 0.05) compared to wild-type animals. In conditions of DPP IV/CD26 deficiency, formation of new capillaries begins earlier and their number was significantly higher (p < 0.05) compared to wild-type animals. Serum DPP IV/CD26 activity in diabetic C57BL/6 animals was reduced the fourth and seventh day of wound healing compared to healthy animals. Conclusion: Obtained results confirmed the hypothesis of the role of DPP IV/CD26 in the process of wound healing in experimental diabetes. Our results indicate an improved process of neovascularization and proliferation of damaged tissue, lower inflammatory response and more successful reepithelization and scar resorption in conditions of DPP IV/CD26 deficiency.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA