Naslov
Association of 1166A>C AT1R, -1562C>T MMP-9, ACE I/D, and CCR5delta32 polymorphisms with abdominal aortic aneurysm in Croatian patients

Autori
Crkvenac Gregorek, Andrea ; Crkvenac Gornik, Kristina ; Stupin Polancec, Darija ; Dabelic, Sanja

Izvornik
Genetic Testing and Molecular Biomarkers (1945-0265) 20 (2016), 10; 616-623

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
abdominal aortic aneurysm ; AT1R ; MMP-9 ; CCR5 ; ACE

Sažetak
Aim: The purpose on this study was to assess the association of four polymorphisms, namely SNP 1166A>C in the angiotensin II type 1 receptor gene (AT1R), SNP -1562C>T in the matrix metalloproteinase-9 gene (MMP-9), the deletion of 32 bp in the chemokine receptor 5 gene (CCR5) and the insertion/deletion of 287 bp in the angiotensin-converting enzyme gene (ACE) with abdominal aortic aneurysm in Croatian patients. Methods: Overall 234 subjects, 117 patients with confirrmed AAA (AAA+) and 117 control subjects (AAA-), were genotyped using PCR or PCR-RFLP analysis. Statistical analyses were performed using MedCalc 12.1 software. Results: The deletion of 287 bp in ACE gene (allele D) was more frequently found among AAA+ patients compared to the AAA- subjects (66.7% vs. 47.9%, p=0.0001), due to higher percentage of DD homozygotes (46.2% vs. 15.4%, p<0.0001), and the increased risk for AAA was detected in both non-adjusted recessive model of inheritance (OR=3.00, 95% CI=1.88-4.79, p=<0.0001), and adjusted for age, sex, smoking, hypertension and hyperlipidemia (OR=4.96 ; 95% CI=1.68- 14.59, p=0.004). The adjusted recessive models also showed increased risk for AAA for the carriers of MMP-9 T allele (OR=15.69, 95% CI= 1.40-175.41, p=0.025). Patients with small aneurysms compared to those with large ones were more frequently carriers of AT1R allele C (37.8% vs. 23.2%, p=0.029), and logistic regression analysis showed decreased risk for developing large aneurysm in both adjusted models, dominant and recessive (OR=0.3929, 95% CI=0.1554-0.9932, p=0.0483 and OR=0.1728, 95% CI=0.0331-0.9023 ; p=0.0374, respectively). No difference among any type of the studied groups or subgroups was observed regarding CCR532 polymorphism. Conclusion: ACE I/D can be associated with AAA, 1166A>C AT1R with the size of the aneurysm, while -1562C>T MMP-9 and CCR532 polymorphism are most probably not associated with AAA in Croatian patients.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Farmacija

POVEZANOST RADA