Naslov
Application of the alkaline Comet assay and the analysis of structural chromosome aberrations in assessment of genetic damage after accidental exposure to ionising radiation.

Autori
Garaj-Vrhovac, Vera ; Kopjar, Nevenka ; Ražem, Dušan ; Vekić, Branko, Miljanić, Saveta ; Ranogajec-Komor, Maria

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of IRPA Regional Congress on Radiation Protection in Central Europe - Radiation Protection and Health / Obelić, B. ; Ranogajec-Komor, M. ; Miljanić, S. ; Krajcar Bronić, I. - Zagreb : Hrvatsko društvo za zaštitu od zračenja, 2002, 3-15

Skup
IRPA Regional Congress on Radiation Protection in Central Europe - Radiation Protection and Health

Mjesto i datum
Dubrovnik, Hrvatska, 20.05.2001. - 25.05.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Cell gel-electrophoresis; white blood-cells; ionizing radiation; human-lymphocytes; individual cells; base damage; exposure; induction; cancer; repair

Sažetak
The effects of lower doses of ionising radiation may not show up for years after exposure and are due to various changes in DNA molecules and chromosomes. Radiation-induced mutations seem to be brought about by the deletion of small pieces of chromosomes during the process of chromosome breakage and repair. Ionising radiation kills rapidly dividing cells, blood lymphocytes among them. The cytogenetical consequences of accidental exposure to g-radiation (radiation dose was 221 mSv) were investigated by using alkaline Comet assay and the analysis of structural chromosomal aberration. Blood samples were collected during one-year period after the incident. By using the Comet assay immediately after accidental exposure a high level of DNA damage was recorded. Although this level was decreasing over a one-year period, it was still elevated compared to normal values of DNA damage for unexposed persons. Immediately after the incident a prevalence of chromosomal over chromatid aberrations was recorded. However, one year after only a few chromatid breaks were recorded. The results confirmed the usefulness of the alkaline Comet assay as a simple and sensitive technique for the biomonitoring of DNA damages, especially in the cases of accidental exposure to ionising radiation.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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