Naslov
Cytokine and chemokine kinetics and their potential as biomarkers in patients with Puumala virus infection

Autori
Svoboda, Petra ; Cvetko Krajinović, Lidija ; Čikeš, Petra ; Topić, Antea ; Bosnar, Martina ; Eraković Haber, Vesna ; Jugović, Davor ; Markotić, Alemka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Program and Abstracts Book - Xth International Conference on HFRS, HPS and Hantaviruses / - Fort Collins (CO), 2016, 73-73

Skup
Xth International Conference on HFRS, HPS and Hantaviruses

Mjesto i datum
Fort Collins (CO), Sjedinjene Američke Države, 31.05.2016. - 03.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Hemorrhagic fever with renal syndrome; clinical picture; Luminex; monocytes; macrophages

Sažetak
Objective: Hemorrhagic fever with renal syndrome (HFRS) is a viral disease caused by hantaviruses, however, it is primarily considered as an immune-mediated disease. Several studies have shown deregulated pro- and anti-inflammatory cytokine/chemokine production during the acute phase of hantavirus infection in sera of HFRS patients. Monocytes/macrophages are important immune cells since they are producing different cytokines/chemokines and are considered one of the target cells for hantaviruses. The aim of our study was to analyze the monocytes/macrophages related cytokines/chemokines profile in sera of HFRS patients infected with Puumala virus (PUUV) according to the severity of their clinical picture. Also, looking at the correlation with some clinical parameters we would like to determine possible cytokines/chemokines as the useful biomarkers for the HFRS prognosis. Methods: The study included 34 HFRS patients with a serologically confirmed PUUV diagnosis, hospitalized in 2014 in Zagreb, Croatia. Patients' serum samples were taken in two acute time points – upon arrival at the hospital and before discharge. The level of PUUV viral load was detected. For analysis of cytokine and chemokine levels Luminex technology of (multiplexed) immunoassay with magnetic beads was used for 16 analytes, namely: IL-1 β, IL-1RA, IL-12(p70), IL-15, IL-18, IL-23, IL-27, MIF, CCL2, CCL3, CCL4, CCL5, CCL7, CCL20, CXCL12a, TGF-β1. Results: The results showed in general, suppression of the early immune response to PUUV i.e. some pro-inflammatory cytokines were down-regulated or absent (IL-1 β, IL-12(p70), IL-23). CCL4, IL-1RA, IL-18 and MIF were elevated in both phases compared to the controls, while CCL5 was downregulated in the first and upregulated in the second phase. In opposite, low levels of IL-15 were recorded in the first serum and not in the second serum sample. Some differences in the measured cytokines/chemokines were detected in patients with mild in comparation to the patients with moderate clinical picture and correlation with several clinical laboratory parameters were observed as well. Conclusions: Some differences in the levels of monocytes/macrophages related cytokines/chemokines in HFRS patients with mild or moderate clinical pictures can be useful biomarkers for the prognosis of the HFRS clinical picture.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti

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