Naslov
Differential requirements of activating and inhibitory Ly49 receptors in recognition of MCMV-infected cells

Autori
Železnjak, Jelena ; Popović, Branka ; Juranić Lisnić, Vanda ; L’Hernault, Anne ; Trautwein, Nico ; Babić Čač, Marina ; Lisnić, Berislav ; Halenius, Anne ; Hengel, Hartmut ; Stevanović, Stefan ; Dölken, Lars ; Krmpotić, Astrid ; Jonjić, Stipan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th European COngress of Immunology Abstract Book / - , 2015

Skup
4th European Congress of Immunology

Mjesto i datum
Beč, Austrija, 06.06.2015. - 09.06.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MCMV; Ly49 receptors; NK cells; immunoevasion

Sažetak
Cytomegalovirus (CMV) has developed multiple immunoevasion strategies to compromise host’s immune response and ensure its survival. We have previously shown that the ligation of an inhibitory Ly49A NK cell receptor to complex of MHC-I and viral protein m04/gp34 leads to a protection of infected cells from specific lysis, impaired proliferation of NK cells and loss of control of viral titer in mice of H-2d and H-2k haplotype early upon an infection (Babić et al. J Exp Med, 2010). Moreover, m04 is essential for the recognition of infected cells by several activating Ly49 receptors (Ly49P, Ly49P1, Ly49D2 and Ly49L), which specifically recognize MHC-I, together with m04 and another viral component (Kielczewska et al. J Exp Med, 2009 ; Pyzik et al. J Exp Med, 2011). Subsequently, we have narrowed down this viral factor to recently described most abundant transcript (MAT) encompassing 2 viral genes, m168-m169 (Juranic Lisnic et al. PLoS Pathog, 2013). It has been shown that MAT transcript encodes poorly translated 17 kDa protein of unknown function and contains a binding site for cellular miR-27. Interestingly, neither function of miR-27 sponge nor the produced protein are responsible for recognition by activating Ly49 receptors, but its 5’UTR. Recently, we have detected additional protein in this region. Furthermore, deletion of MAT region of MCMV results in attenuation of the virus in vivo. We have also showed that absence of MAT results in dramatic changes of MHC-I maturation and host and viral peptide repertoire. However, the underlying mechanisms are not completely elucidated due to numerous functions MAT performs in the infected cells. Specifically, our current efforts are aimed to define how 5’UTR mediates Ly49 recognition.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA