Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 819426

Purification of mumps and measles virus by hydrophobic interaction chromatography on monolithic columns


Sviben, Dora; Forčić, Dubravko; Ivančić-Jelečki, Jelena; Halassy, Beata; Brgles, Marija
Purification of mumps and measles virus by hydrophobic interaction chromatography on monolithic columns // 7th Monolith Summer School & Symposium : Book of Abstracts
Portorož, Slovenija, 2016. str. 20-20 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 819426 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Purification of mumps and measles virus by hydrophobic interaction chromatography on monolithic columns

Autori
Sviben, Dora ; Forčić, Dubravko ; Ivančić-Jelečki, Jelena ; Halassy, Beata ; Brgles, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th Monolith Summer School & Symposium : Book of Abstracts / - , 2016, 20-20

Skup
Monolith Summer School & Symposium (7 ; 2016)

Mjesto i datum
Portorož, Slovenija, 29.05.2016. - 01.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Mumps virus ; Measles virus ; Hydrophobic interaction chromatography ; Monoliths ; Nanoparticle Tracking Analysis

Sažetak
Mumps (MuV) and measles viruses (MeV) are nonsegmented, negative stranded RNA viruses of the family Paramyxoviridae, subfamily Paramyxovirinae. MuV and MeV are the cause of mumps and measles disease, both preventable by vaccination. Purity of virus particles used for vaccines or gene therapy is of utmost importance in order to obtain a product with high purity that can meet the stringent guidelines of the regulatory authorities for potency and safety. Chromatography is being increasingly used in virus purification, especially since the development of new chromatographic matrices. Here, research on MuV and MeV purification by hydrophobic interaction chromatography (HIC) on monolithic column is presented. The influence of different ammonium sulfate concentrations in the binding buffer on purification efficacy as well as column channel size was examined. Recovery of viable viruses was determined by 50% cell culture infective dose assay. Size and concentration of total virus particles were determined by Nanoparticle Tracking Analysis using NanoSight instrument and elution fractions were tested for Vero cell proteins by ELISA. Results show that total particle concentration, as well as the concentration of live viruses increases with decreasing ammonium sulfate concentration in the elution buffer. High ammonium sulfate concentrations did not induce virus aggregation but did induce some loss of viable particles. Decrease of MuV and MeV titers was also observed upon loading the virus on chromatographic system without the column. Viable virus recovery was moderately high (up to 40 %), therefore HIC appears to be a promising method for purification of viable MeV and MuV.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
HRZZ-UIP-2013-11-8193 - Kromatografsko pročišćavanje biomolekula i njihova karakterizacija (CHROBIO) (Brgles, Marija, HRZZ - 2013-11) ( CroRIS)

Ustanove:
Sveučilište u Zagrebu


Citiraj ovu publikaciju:

Sviben, Dora; Forčić, Dubravko; Ivančić-Jelečki, Jelena; Halassy, Beata; Brgles, Marija
Purification of mumps and measles virus by hydrophobic interaction chromatography on monolithic columns // 7th Monolith Summer School & Symposium : Book of Abstracts
Portorož, Slovenija, 2016. str. 20-20 (poster, međunarodna recenzija, sažetak, znanstveni)
Sviben, D., Forčić, D., Ivančić-Jelečki, J., Halassy, B. & Brgles, M. (2016) Purification of mumps and measles virus by hydrophobic interaction chromatography on monolithic columns. U: 7th Monolith Summer School & Symposium : Book of Abstracts.
@article{article, author = {Sviben, Dora and For\v{c}i\'{c}, Dubravko and Ivan\v{c}i\'{c}-Jele\v{c}ki, Jelena and Halassy, Beata and Brgles, Marija}, year = {2016}, pages = {20-20}, keywords = {Mumps virus, Measles virus, Hydrophobic interaction chromatography, Monoliths, Nanoparticle Tracking Analysis}, title = {Purification of mumps and measles virus by hydrophobic interaction chromatography on monolithic columns}, keyword = {Mumps virus, Measles virus, Hydrophobic interaction chromatography, Monoliths, Nanoparticle Tracking Analysis}, publisherplace = {Portoro\v{z}, Slovenija} }
@article{article, author = {Sviben, Dora and For\v{c}i\'{c}, Dubravko and Ivan\v{c}i\'{c}-Jele\v{c}ki, Jelena and Halassy, Beata and Brgles, Marija}, year = {2016}, pages = {20-20}, keywords = {Mumps virus, Measles virus, Hydrophobic interaction chromatography, Monoliths, Nanoparticle Tracking Analysis}, title = {Purification of mumps and measles virus by hydrophobic interaction chromatography on monolithic columns}, keyword = {Mumps virus, Measles virus, Hydrophobic interaction chromatography, Monoliths, Nanoparticle Tracking Analysis}, publisherplace = {Portoro\v{z}, Slovenija} }




Contrast
Increase Font
Decrease Font
Dyslexic Font