Naslov
Glucose is stable during prolonged storage in un-centrifuged Greiner tubes with liquid citrate buffer, but not in serum and NaF/KOx tubes

Autori
Juričić, Gordana ; Bakliža, Ana ; Saračević, Andrea ; Milevoj Kopčinović, Lara ; Dobrijević, Sanja ; Drmić, Sandra ; Šimundić, Ana-Maria

Izvornik
Clinical chemistry and laboratory medicine (1434-6621) 54 (2016), 3; 411-418

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
diabetes ; Glucomedics ; glycolysis ; glycolysis inhibitors ; preanalytical phase

Sažetak
Background: Delayed sample processing can affect accurate glucose measurement. Our aim was to investigate the stability of glucose in samples collected in serum, sodium fluoride/potassium oxalate (NaF/KOx) and Glucomedics tubes processed according to different controlled pre-centrifugation delays (up to 180 min after venipuncture) in order to simulate prolonged sample transport between venipuncture and centrifugation. Methods: Samples were collected from healthy volunteers (n=80) into either serum or NaF/KOx and Glucomedics tubes. Glucose concentration was measured in samples centrifuged immediately after venipuncture and compared with tubes processed with a delay of 60, 120 and 180 min prior to centrifugation. Differences between baseline and respective delayed centrifugation glucose value for each tube type were tested using the paired t-test. Mean bias calculated for each tube type and delay protocol was compared to recommended quality specifications for glucose (2.2%). Results: Glucose concentrations measured in all three delayed tube types were lower in comparison to respective baseline glucose concentrations measured in immediately processed tube (p<0.001). The highest decrease in glucose was observed in serum tubes in all specified time points (p<0.001), while glucose was most stable in Glucomedics tubes (p<0.001). The decrease in glucose observed for serum and NaF/KOx tubes was clinically significant at all specified time points while the bias for Glucomedics tubes did not exceed the criteria even with a centrifugation delay of 180 min. Conclusions: Glucose stability in un- centrifuged Glucomedics tubes is much superior to serum and NaF/KOx tubes. Glucomedics tubes can be left un-centrifuged for up to 3 h without affecting glucose concentration.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA