Pregled bibliografske jedinice broj: 81414
A Mild and Efficient Solid-Support Synthesis of Novel Oligonucleotide Conjugates
A Mild and Efficient Solid-Support Synthesis of Novel Oligonucleotide Conjugates // Bioconjugate Chemistry, 9 (1998), 2; 283-291 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 81414 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
A Mild and Efficient Solid-Support Synthesis of Novel Oligonucleotide Conjugates
Autori
Habuš, Ivan ; Xie, Jin ; Iyer, Radhakrishnan P. ; Zhou, Wen-Qiang ; Shen, Ling X. ; Agrawal, S.
Izvornik
Bioconjugate Chemistry (1043-1802) 9
(1998), 2;
283-291
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Oligonucleotide conjugate synthesis
Sažetak
Conjugates of oligodeoxyribonucleotide phosphorothioate (ODN-PS) with folic acid, retinoic acid, arachidonic acid, and methoxypoly(ethylene glycol)propionic acid have been synthesized. The procedure involved the initial solid-phase preparation of 5'-amino-functionalized ODN-PS using N-pent-4-enoyl-derived (PNT) nucleoside phosphoramidites followed by conjugation of the oligonucleotide either to the ligand acids, using 1-/3-(dimethylamino)propyl/-3-ethylcarbodiimide as a coupling reagent, or to their corresponding succinimidyl derivatives. Subsequent exposure of the support to aqueous ammonium hydrohide (28%, 2h, 55oC) resulted in the release of the fully deprotected ODN conjugates, which were purified by reversed-phase HPLC or by preparative polyacrylamide gel electrophoresis. The identity of the oligonucleotide conjugates was confirmed by MALDI-TOF mass spectral analysis.
Izvorni jezik
Engleski
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
Uključenost u ostale bibliografske baze podataka::
- Chemical Abstracts
